公开(公告)号：US20150353967A1

公开(公告)日：2015-12-10

申请号：US14760217

申请日：2014-01-13

申请人： Alan Gideon WERKER ,  Fernando MORGAN-SAGASTUME ,  Lamija KARABEGOVIC ,  Simon Olof Harald BENGTSSON ,  Francesco VALENTINO ,  Mauro MAJONE ,  VEOLIA WATER SOLUTIONS & TECHNOLOGIES sUPPORT

发明人： Alan Gideon WERKER ,  Fernando MORGAN-SAGASTUME ,  Lamija KARABEGOVIC ,  Simon Olof Harald BENGTSSON ,  Francesco VALENTINO ,  Mauro MAJONE

IPC分类号： C12P7/62 ,  C02F3/12

CPC分类号： C12P7/625 ,  C02F3/006 ,  C02F3/12 ,  C02F11/02 ,  C02F2209/21 ,  C02F2305/06

摘要： This disclosure relates to methods for producing a PHA-rich-biomass from open mixed cultures. Mixed liquor containing biomass is directed into a fed-batch reactor. The reactor includes at least one biomass stimulating zone and at least one biomass maintenance zone. A feed containing RBCOD, bioavailable N, and bioavailable P is directed into the fed-batch reactor. The respiration rate of at least a portion of the biomass is intermittently and repeatedly stimulated in the stimulating zone by directing the feed into the fed-batch reactor and exposing the biomass to a relatively high concentration of RBCOD. The biomass is then transferred to the maintenance zone, where the biomass is exposed to a relatively low concentration of RBCOD. Thereafter, the biomass is circulated back and forth between the stimulating zone and the maintenance zone. Throughout the methods, the concentration of N and/or P relative to the RBCOD in the feed is controlled by controlling the ratio of N to RBCOD and/or the ration of P to RBCOD in the feed.

摘要翻译： 本公开涉及从开放混合培养物生产富含PHA的生物质的方法。 含有生物质的混合液被引入补料分批反应器。 反应器包括至少一个生物质刺激区和至少一个生物质维持区。 将含有RBCOD，生物可利用的N和生物可利用的P的饲料引入补料分批反应器。 通过将饲料引入补料分批反应器并将生物量暴露于较高浓度的RBCOD，在刺激区中间歇地和重复地刺激至少一部分生物质的呼吸速率。 然后将生物量转移到维护区，其中生物量暴露于较低浓度的RBCOD。 此后，生物质在刺激区和维护区之间来回循环。 在整个方法中，通过控制饲料中N与RBCOD的比例和/或P与RBCOD的比例来控制饲料中N和/或P相对于RBCOD的浓度。