公开(公告)号：US5565328A

公开(公告)日：1996-10-15

申请号：US579089

申请日：1995-12-27

申请人： Shoshana Bascomb ,  Frank J. Swenson ,  Ted Sand

发明人： Shoshana Bascomb ,  Frank J. Swenson ,  Ted Sand

IPC分类号： C12Q1/04 ,  C12Q1/26 ,  H01L21/306

CPC分类号： C12Q1/04 ,  Y10S435/968

摘要： The present invention relates to a method to determine the concentration of an unknown substance in a colorimetric reaction using a fluorometric reader. The change in color can be monitored by observing the change of fluorescence of a known amount of a suitable fluorophore in the reaction chamber. Under appropriate conditions the absorption spectrum of the chromophore overlaps with the emission spectrum of the fluorophore thereby allowing the change in fluorescence to be proportional to the intensity of color in the reaction and thus, proportional to the quantity of the substance of interest. Specifically, 8-methoxypyrene tri-sulfonic acid is used as the fluorophore.

摘要翻译： 本发明涉及使用荧光读数器确定比色反应中未知物质的浓度的方法。 可以通过观察已知量的合适的荧光团在反应室中的荧光变化来监测颜色变化。 在合适的条件下，发色团的吸收光谱与荧光团的发射光谱重叠，从而允许荧光的变化与反应中颜色的强度成比例，因此与感兴趣的物质的数量成比例。 具体地说，使用8-甲氧基芘三磺酸作为荧光团。