公开(公告)号：US20050069979A1

公开(公告)日：2005-03-31

申请号：US10497123

申请日：2002-11-26

申请人： Steffen Zeng ,  Franz-Markus Bogner ,  Renate Kunert ,  Dethardt Mueller ,  Florian Unterluggauer

发明人： Steffen Zeng ,  Franz-Markus Bogner ,  Renate Kunert ,  Dethardt Mueller ,  Florian Unterluggauer

IPC分类号： C12N15/09 ,  C07K14/505 ,  C12N5/10 ,  C12P21/02 ,  C12N15/85

CPC分类号： C12P21/02 ,  C07K14/505 ,  C12N2500/34 ,  C12N2500/76 ,  C12N2500/92 ,  C12N2510/02

摘要： The invention provides a method for producing a recombinant polypeptide of interest which method comprises: (a) providing a host cell which comprises a nucleotide sequence which encodes the recombinant polypeptide of interest and which directs expression of the recombinant polypeptide of interest in the host cell; (b) providing a serum-free culture medium which comprises (i) water, a plant-derived peptone, an osmolality regulator, a buffer, an energy source, at least one amino acid, a lipid source or precursor, a source of iron, non-ferrous metal ions and optionally one or more vitamins and cofactors; and (ii) does not contain any full-length polypeptides; and (c) culturing the host cell in the culture medium under conditions that allow for expression of the recombinant polypeptide of interest.

摘要翻译： 本发明提供了一种用于产生感兴趣的重组多肽的方法，所述方法包括：（a）提供宿主细胞，其包含编码所述重组多肽的核苷酸序列，其指导所述重组感兴趣多肽在所述宿主细胞中的表达; （b）提供无血清培养基，其包含（i）水，植物来源的蛋白胨，渗透压调节剂，缓冲液，能量源，至少一种氨基酸，脂质源或前体，铁源 ，有色金属离子和任选的一种或多种维生素和辅因子; 和（ii）不含任何全长多肽; 和（c）在允许感兴趣的重组多肽表达的条件下在培养基中培养宿主细胞。