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1.发明授权Carboxy terminal residues as predictors and regulators of protein stability 有权羧基末端残基作为蛋白质稳定性的预测因子和调节物公开(公告)号：US08268581B2
公开(公告)日：2012-09-18
申请号：US12431315
申请日：2009-04-28
申请人： Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
发明人： Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
CPC分类号： C12N9/0071 , C07K2319/00 , C07K2319/21 , C07K2319/31 , C07K2319/41 , C07K2319/60 , C12Y114/19001
摘要： Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in Mycobacterium smegmatis. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.
摘要翻译： 提供了可用于预测和控制表达多肽的稳定性的组合物和方法。 组合物和方法可以用于预测和按需要增加或降低在分枝杆菌中重组表达的蛋白质的稳定性，例如在耻垢分枝杆菌中表达的DesA3。 在C末端和倒数第二的位置，可以使用带有带侧链，大非极性侧链或无侧链的残基取代以降低或抑制蛋白质降解。 在C末端的最初位置，没有侧链或酸性侧链的残基可以增加稳定性，即降低或抑制蛋白质降解。 仅在多肽的最后3个残基的组合取代可以使多肽在分枝杆菌宿主的异源表达期间更稳定。
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2.发明申请CARBOXY TERMINAL RESIDUES AS PREDICTORS AND REGULATORS OF PROTEIN STABILITY 有权作为蛋白质稳定性的预测因子和调节因子的羧基末端残基公开(公告)号：US20120107904A1
公开(公告)日：2012-05-03
申请号：US12431315
申请日：2009-04-28
申请人： Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
发明人： Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
CPC分类号： C12N9/0071 , C07K2319/00 , C07K2319/21 , C07K2319/31 , C07K2319/41 , C07K2319/60 , C12Y114/19001
摘要： Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in Mycobacterium smegmatis. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.
摘要翻译： 提供了可用于预测和控制表达多肽的稳定性的组合物和方法。 组合物和方法可以用于预测和按需要增加或降低在分枝杆菌中重组表达的蛋白质的稳定性，例如在耻垢分枝杆菌中表达的DesA3。 在C末端和倒数第二的位置，可以使用带有带侧链，大非极性侧链或无侧链的残基取代以降低或抑制蛋白质降解。 在C末端的最初位置，没有侧链或酸性侧链的残基可以增加稳定性，即降低或抑制蛋白质降解。 仅在多肽的最后3个残基的组合取代可以使多肽在分枝杆菌宿主的异源表达期间更稳定。
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