公开(公告)号：US20110038923A1

公开(公告)日：2011-02-17

申请号：US12513006

申请日：2007-11-01

申请人： Gary Weisinger ,  Rona Limor ,  Naftali Stern

发明人： Gary Weisinger ,  Rona Limor ,  Naftali Stern

IPC分类号： A61K9/127 ,  C12N15/63 ,  C12N5/071 ,  A61K31/7088 ,  C12N5/02 ,  A61P3/04

CPC分类号： C12N15/1137 ,  A61K48/0058 ,  A61K48/0066 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2320/32 ,  C12N2330/51

摘要： The present invention is directed to constructs, compositions and methods for modulating platelet-type 12 lipoxygenase (12-LO) in adipose tissue in vivo. Specifically, the invention provides constructs encoding expression-inhibiting oligonucleic acids, e.g., antisense and RNA interfering (RNAi) molecules, targeted to a platelet-type 12-LO gene or a transcript thereof, which are capable of reducing or silencing platelet-type 12-LO expression specifically in adipocytes and pre-adipocytes. Vectors of these constructs (including, but not limited to, viral vectors), compositions of them and methods of using same for the treatment and amelioration of conditions associated with excess fat cell mass and obesity are also provided.

摘要翻译： 本发明涉及在体内脂肪组织中调节血小板型12脂氧合酶（12-LO）的构建体，组合物和方法。 具体地，本发明提供编码表达抑制性寡核酸的构建体，例如靶向血小板型12-LO基因的反义和RNA干扰（RNAi）分子或其转录物，其能够减少或沉默血小板型12 -LO在脂肪细胞和前脂肪细胞中的表达。 还提供了这些构建体（包括但不限于病毒载体）的载体，它们的组合物及其使用方法用于治疗和改善与过量脂肪细胞团块和肥胖相关的病症。

公开(公告)号：US09279127B2

公开(公告)日：2016-03-08

申请号：US12513006

申请日：2007-11-01

申请人： Gary Weisinger ,  Rona Limor ,  Naftali Stern

发明人： Gary Weisinger ,  Rona Limor ,  Naftali Stern

IPC分类号： C07H21/04 ,  A61K31/70 ,  C12N15/63 ,  C12N15/113 ,  A61K48/00

CPC分类号： C12N15/1137 ,  A61K48/0058 ,  A61K48/0066 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2320/32 ,  C12N2330/51

摘要： The present invention is directed to constructs, compositions and methods for modulating platelet-type 12 lipoxygenase (12-LO) in adipose tissue in vivo. Specifically, the invention provides constructs encoding expression-inhibiting oligonucleic acids, e.g., antisense and RNA interfering (RNAi) molecules, targeted to a platelet-type 12-LO gene or a transcript thereof, which are capable of reducing or silencing platelet-type 12-LO expression specifically in adipocytes and pre-adipocytes. Vectors of these constructs (including, but not limited to, viral vectors), compositions of them and methods of using same for the treatment and amelioration of conditions associated with excess fat cell mass and obesity are also provided.

摘要翻译： 本发明涉及在体内脂肪组织中调节血小板型12脂氧合酶（12-LO）的构建体，组合物和方法。 具体地，本发明提供编码表达抑制性寡核酸的构建体，例如靶向血小板型12-LO基因的反义和RNA干扰（RNAi）分子或其转录物，其能够减少或沉默血小板型12 -LO在脂肪细胞和前脂肪细胞中的表达。 还提供了这些构建体（包括但不限于病毒载体）的载体，它们的组合物及其使用方法用于治疗和改善与过量脂肪细胞团块和肥胖相关的病症。

公开(公告)号：US6106827A

公开(公告)日：2000-08-22

申请号：US862438

申请日：1997-05-24

申请人： Edmund F. La Gamma ,  Gary Weisinger ,  Robert E. Strecker ,  Nicholas J. Lenn

发明人： Edmund F. La Gamma ,  Gary Weisinger ,  Robert E. Strecker ,  Nicholas J. Lenn

IPC分类号： A61K48/00 ,  C12N5/08 ,  C12N9/02 ,  C12N9/12 ,  C12N15/85 ,  C12N5/06 ,  C12N15/63

CPC分类号： C12N9/0071 ,  A61K48/0058 ,  C12N15/85 ,  C12N9/1211 ,  C12Y114/13041 ,  C12Y114/16002 ,  A61K48/00 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/85

摘要： A genetically modified astrocyte for gene therapy is provided. The genetically modified astrocyte includes one or more stably introduced DNA sequences selected from DNA encoding a selectable marker, DNA encoding a poison pill, and DNA encoding a molecule useful for gene therapy. The genetically modified astrocyte may be produced utilizing plasmids and non-viral transfection methods, as are also provided by the subject invention. Methods for producing and utilizing the genetically modified astrocytes and regulating the engineered products, as well as kits thereof, are further provided.

摘要翻译： 提供用于基因治疗的转基因星形胶质细胞。 遗传修饰的星形胶质细胞包括选自编码选择性标记的DNA，编码毒药的DNA和编码可用于基因治疗的分子的DNA的一种或多种稳定导入的DNA序列。 基因修饰的星形胶质细胞可以利用本发明也提供的质粒和非病毒转染方法产生。 进一步提供生产和利用转基因星形胶质细胞并调节工程化产品的方法及其试剂盒。