公开(公告)号：US20240294919A1

公开(公告)日：2024-09-05

申请号：US18563140

申请日：2022-05-25

申请人： KENT STATE UNIVERSITY

发明人： Soumitra BASU ,  Nathan BEALS ,  Mohamed M. FARHATH ,  Prakash KHAREL

IPC分类号： C12N15/113 ,  A61K47/69 ,  A61P25/16

CPC分类号： C12N15/1137 ,  A61K47/6923 ,  A61K47/6929 ,  A61P25/16 ,  C12N2310/151 ,  C12N2310/3513 ,  C12N2320/32 ,  C12Y114/16002

摘要： Novel DNA molecule-based compositions and methods for delivery to target cells provide modulation of target G-quadruplex formation in the tyrosine hydroxylase (TH) promoter and allow for enhancement or repressing of specific G-quadruplexes that in turn regulate tyrosine hydroxylase transcription and catecholamine production, specifically dopamine. Use of the DNA-molecule-based compositions in treatment of neurological diseases and disorders is facilitated through a nanoparticle-based delivery system.

公开(公告)号：US09233999B2

公开(公告)日：2016-01-12

申请号：US14023537

申请日：2013-09-11

申请人： Hung-Chien Chien

发明人： Hung-Chien Chien

IPC分类号： A61K38/00 ,  A61K38/03 ,  C07K2/00 ,  C12N9/02

CPC分类号： C07K2/00 ,  C12N9/0071 ,  C12Y114/16002

摘要： The present invention provides a method of biosynthesizing a recombinant protein containing the biologically active peptide, the pharmaceutical composition containing the recombinant protein as well as the preparation of the recombinant protein. Simply speaking the consecutively multiple copies of the bioactive peptide are replaced in the amino acid sequence of a recombinant protein (so called peptide-protein). Then, a high concentration and high yield of the peptide-protein containing the consecutively multiple copies of the bioactive peptide is produced by the biosafety and edible strain of yeast without any endotoxin contamination.

摘要翻译： 本发明提供了生物合成含有生物活性肽的重组蛋白质的方法，含有重组蛋白质的药物组合物以及重组蛋白质的制备。 简言之，在重组蛋白质（所谓的肽 - 蛋白质）的氨基酸序列中连续复制多个生物活性肽的拷贝。 然后，通过酵母的生物安全和可食用菌株产生含有连续多个生物活性肽拷贝的肽 - 蛋白质的高浓度和高收率，而没有任何内毒素污染。

公开(公告)号：US20030027779A1

公开(公告)日：2003-02-06

申请号：US10057777

申请日：2002-01-25

申请人： Spinal Cord Society

发明人： Toomas Neuman ,  Kikuo Suda ,  Howard O. Nornes

IPC分类号： A61K048/00 ,  A61K009/127 ,  C12N015/88

CPC分类号： C07K14/005 ,  C07K14/4738 ,  C07K14/48 ,  C12N9/0071 ,  C12N15/85 ,  C12N2710/10322 ,  C12Y114/13041 ,  C12Y114/16002

摘要： A method is provided for inducing DNA synthesis in differentiated neurons. According to certain embodiments of the invention, a method for inducing DNA synthesis in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a differentiated neuron, and transfecting a differentiated neuron with the vector. According to certain embodiments of the invention, a method for integrating DNA encoding a desired protein in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a neuron, obtaining DNA encoding a desired protein, and cotransfecting a differentiated neuron with the vector and the DNA encoding the desired protein such that the DNA encoding the desired protein is integrated in the differentiated neuron and the desired protein is produced.

公开(公告)号：US06458529B1

公开(公告)日：2002-10-01

申请号：US08459994

申请日：1995-06-02

申请人： Axel Kahn ,  Gildas Le Gal la Salle ,  Jacques Mallet ,  Michel Perricaudet ,  Marc Peschanski ,  Jean-Jacques Robert

发明人： Axel Kahn ,  Gildas Le Gal la Salle ,  Jacques Mallet ,  Michel Perricaudet ,  Marc Peschanski ,  Jean-Jacques Robert

IPC分类号： C12Q168

CPC分类号： C12N15/86 ,  A61K38/185 ,  A61K38/44 ,  A61K38/47 ,  A61K48/00 ,  C12N9/0006 ,  C12N2710/10343 ,  C12N2830/008 ,  C12Y114/16002 ,  C12Y302/01051

摘要： The invention concerns a recombinant DNA vector characterized in that it is capable of directing the expression an/or transcription of a selected nucleotide sequence in the cells of the central nervous system and in that it comprises (i) at least part of the genome of an adenovirus, including the regions required for that adenovirus to penetrate into the cells normally infectable by that adenovirus and (ii) being inserted into said part of genome of an adenovirus under the control of a promoter, either present or also inserted into said genome part and operative in said cells. This recombinant vector finds particular use in the treatment of diseases of the central nervous system, also in gene therapy.

摘要翻译： 本发明涉及一种重组DNA载体，其特征在于其能够指导所选择的核苷酸序列在中枢神经系统的细胞中的表达/转录，并且其包含（i）至少部分基因组 腺病毒，其包括该腺病毒所需的区域穿透通常可被该腺病毒感染的细胞，和（ii）插入在启动子控制下的，存在于或也插入到所述基因组部分中的腺病毒的基因组的所述部分基因组中， 在所述细胞中起作用。 该重组载体特别用于治疗中枢神经系统疾病，也用于基因治疗。

公开(公告)号：US06372721B1

公开(公告)日：2002-04-16

申请号：US09408508

申请日：1999-09-30

申请人： Toomas Neuman ,  Kikuo Suda ,  Howard O. Nornes

发明人： Toomas Neuman ,  Kikuo Suda ,  Howard O. Nornes

IPC分类号： A61K317088

CPC分类号： C07K14/005 ,  C07K14/4738 ,  C07K14/48 ,  C12N9/0071 ,  C12N15/85 ,  C12N2710/10322 ,  C12Y114/13041 ,  C12Y114/16002

摘要： A method is provided for inducing DNA synthesis in differentiated neurons. According to certain embodiments of the invention, a method for inducing DNA synthesis in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a differentiated neuron, and transfecting a differentiated neuron with the vector. According to certain embodiments of the invention, a method for integrating DNA encoding a desired protein in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a neuron, obtaining DNA encoding a desired protein, and cotransfecting a differentiated neuron with the vector and the DNA encoding the desired protein such that the DNA encoding the desired protein is integrated in the differentiated neuron and the desired protein is produced.

摘要翻译： 提供了一种诱导分化神经元DNA合成的方法。 根据本发明的某些实施方案，提供了在分化神经元中诱导DNA合成的方法，其包括获得包含编码E2F调节子和/或E1A调节子的核酸的载体，其中所述载体可用于表达所述核酸 在分化神经元中，并用载体转染分化的神经元。 根据本发明的某些实施方案，提供了一种用于整合在分化神经元中编码所需蛋白质的DNA的方法，其包括获得包含编码E2F调节剂和/或E1A调节剂的核酸的载体，其中所述载体可用于表达 神经元中的核酸，获得编码期望蛋白质的DNA，并用载体和编码所需蛋白质的DNA共转染分化的神经元，使得编码期望的蛋白质的DNA整合在分化的神经元和所需的蛋白质中。

公开(公告)号：US5753491A

公开(公告)日：1998-05-19

申请号：US467958

申请日：1995-06-06

申请人： Eugene O. Major ,  Carlo S. Tornatore ,  Gal Yadid

发明人： Eugene O. Major ,  Carlo S. Tornatore ,  Gal Yadid

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/12 ,  A61K35/30 ,  A61K35/54 ,  A61K38/44 ,  A61K48/00 ,  A61P25/28 ,  C12N5/073 ,  C12N5/079 ,  C12N5/10 ,  C12N9/02 ,  C12N15/52 ,  C12N15/85 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12N9/0071 ,  A61K35/30 ,  A61K35/54 ,  A61K48/00 ,  C12N15/85 ,  C12N5/0622 ,  C12Y114/13041 ,  C12Y114/16002 ,  A61K2035/126 ,  A61K38/00 ,  C12N2510/04 ,  C12N2800/108 ,  C12N2830/42

摘要： The present invention generally relates to methods for treating a host by implanting genetically unrelated cells in the host. More particularly, the present invention provides human fetal neuro-derived cell lines, and methods of treating a host by implantation of these immortalized human fetal neuro-derived cells into the host.

摘要翻译： 本发明一般涉及通过在宿主中植入遗传上不相关的细胞来治疗宿主的方法。 更具体地，本发明提供人胎儿神经衍生的细胞系，以及通过将这些永生化的人胎儿神经衍生细胞植入宿主来治疗宿主的方法。

公开(公告)号：US11866749B2

公开(公告)日：2024-01-09

申请号：US17079010

申请日：2020-10-23

申请人： Danmarks Tekniske Universitet

发明人： Hao Luo ,  Jochen Förster

IPC分类号： C12N9/88 ,  C12N9/78 ,  C12N9/02 ,  C12P17/10 ,  C12P13/22 ,  C12P7/22 ,  C12N1/16 ,  C12N1/14 ,  C12N1/20

CPC分类号： C12N9/78 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0071 ,  C12N9/88 ,  C12P7/22 ,  C12P13/225 ,  C12P13/227 ,  C12P17/10 ,  C12Y114/16001 ,  C12Y114/16002 ,  C12Y114/16004 ,  C12Y305/04016 ,  C12Y402/01096

摘要： Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.

公开(公告)号：US06756523B1

公开(公告)日：2004-06-29

申请号：US08460478

申请日：1995-06-02

申请人： Axel Kahn ,  Gildas Le Gal La Salle ,  Jacques Mallet ,  Michel Perricaudet ,  Marc Peschanski ,  Jean-Jacques Robert

发明人： Axel Kahn ,  Gildas Le Gal La Salle ,  Jacques Mallet ,  Michel Perricaudet ,  Marc Peschanski ,  Jean-Jacques Robert

IPC分类号： A01K6700

CPC分类号： C12N15/86 ,  A61K38/185 ,  A61K38/44 ,  A61K38/47 ,  A61K48/00 ,  C12N9/0006 ,  C12N2710/10343 ,  C12N2830/008 ,  C12Y114/16002 ,  C12Y302/01051

摘要： The invention concerns a recombinant DNA vector characterized in that it is capable of directing the expression an/or transcription of a selected nucleotide sequence in the cells of the central nervous system and in that it comprises (i) at least part of the genome of an adenovirus, including the regions required for that adenovirus to penetrate into the cells normally infectable by that adenovirus and (ii) being inserted into said part of genome of an adenovirus under the control of a promoter, either present or also inserted into said genome part and operative in said cells. This recombinant vector finds particular use in the treatment of diseases of the central nervous system, also in gene therapy.

摘要翻译： 本发明涉及一种重组DNA载体，其特征在于其能够指导所选择的核苷酸序列在中枢神经系统的细胞中的表达/转录，并且其包含（i）至少部分基因组 腺病毒，其包括该腺病毒所需的区域穿透通常可被该腺病毒感染的细胞，和（ii）插入在启动子控制下的，存在于或也插入到所述基因组部分中的腺病毒的基因组的所述部分基因组中， 在所述细胞中起作用。 该重组载体特别用于治疗中枢神经系统疾病，也用于基因治疗。

公开(公告)号：US20020110546A1

公开(公告)日：2002-08-15

申请号：US09973198

申请日：2001-10-10

申请人： Pro-Virus, Inc.

发明人： Eugene O. Major ,  Carlo S. Tornatore ,  Kris Bankiewicz

IPC分类号： A61K048/00

CPC分类号： C12N9/0071 ,  A61K35/30 ,  A61K35/54 ,  A61K38/00 ,  A61K48/00 ,  A61K2035/126 ,  C12N5/0622 ,  C12N15/85 ,  C12N2510/04 ,  C12Y114/13041 ,  C12Y114/16002 ,  Y10S435/948

摘要： Human fetal neuro-derived cell lines are implanted into host tissues. The methods allow for treatment of a variety of neurological disorders and other diseases. A preferred cell line is SVG.

摘要翻译： 将人胎儿神经衍生的细胞系植入宿主组织。 该方法允许治疗各种神经障碍和其他疾病。 优选的细胞系是SVG。

公开(公告)号：US5981165A

公开(公告)日：1999-11-09

申请号：US482079

申请日：1995-06-07

申请人： Samuel Weiss ,  Brent Reynolds

发明人： Samuel Weiss ,  Brent Reynolds

IPC分类号： C12Q1/00 ,  A61K35/12 ,  A61K35/30 ,  A61K38/17 ,  A61K38/18 ,  A61K38/44 ,  A61K48/00 ,  A61P25/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N5/071 ,  C12N5/0793 ,  C12N5/0797 ,  C12N9/08 ,  C12N9/38 ,  C12Q1/02 ,  C12N5/00 ,  A61K38/30

CPC分类号： C12N5/0619 ,  A61K35/30 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N5/0618 ,  C12N5/0623 ,  C12N5/0676 ,  C12N9/0071 ,  C12Y114/16002 ,  A01K2217/05 ,  A61K35/12 ,  A61K48/00 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2502/08 ,  C12N2510/00

摘要： A culture method for inducing the expression of tyrosine hydroxylase in neural cells is provided. Mammalian CNS neural cells are cultured in the presence of a fibroblast growth factor and at least one selected from a member of the transforming growth factor beta family, a feeder layer bed of cells, and cell conditioned medium. Cells cultured as provided above may be transplanted to provide dopaminergic cells to a patient. The cells may also be used in methods for drug screening.

摘要翻译： 提供了诱导神经细胞中酪氨酸羟化酶表达的培养方法。 哺乳动物CNS神经细胞在成纤维细胞生长因子和选自转化生长因子β家族成员，细胞饲养层床和细胞条件培养基中的至少一种的存在下培养。 可以移植如上所述培养的细胞，以向患者提供多巴胺能细胞。 细胞也可用于药物筛选的方法中。