Liquid reagent to detect creatine kinase
    2.
    发明授权
    Liquid reagent to detect creatine kinase 有权
    液体试剂检测肌酸激酶

    公开(公告)号:US06306617B1

    公开(公告)日:2001-10-23

    申请号:US09468416

    申请日:1999-12-20

    IPC分类号: C12Q150

    CPC分类号: C12Q1/50

    摘要: A liquid reagent for detecting creatine kinase activity. The liquid reagent includes G6PDH, hexokinase, creatine phosphate, glucose, ADP, NAD(P) and thioglycerol, which are split into two separate batches of reagents for storage. G6PDH is contained in one batch, and thioglycerol together with a further substance is contained in the other batch. The batches of reagents are mixed together prior to use for detection purposes.

    摘要翻译: 用于检测肌酸激酶活性的液体试剂。 液体试剂包括G6PDH,己糖激酶,磷酸肌酸,葡萄糖,ADP,NAD(P)和硫甘油,分成两批分开的试剂储存。 G6PDH包含在一批中,另一批含有硫甘油与另外的物质。 在使用前将批次的试剂混合在一起用于检测目的。

    IMMUNOASSAY METHOD
    3.
    发明申请
    IMMUNOASSAY METHOD 审中-公开
    免疫方法

    公开(公告)号:US20070243559A1

    公开(公告)日:2007-10-18

    申请号:US11695885

    申请日:2007-04-03

    IPC分类号: G01N33/53

    CPC分类号: G01N33/5306 G01N33/54393

    摘要: An immunoassay method including the steps of: preparing a measuring sample by mixing a specimen with an immunoreactive substance, capable of specifically binding to a defined analyte, allowing the sample to react for a given reaction time, measuring a signal related to interactions of the immunoreactive substance with the analyte in the sample over the reaction time, checking on the basis of signals measured at different times, whether or not prozone effect has occurred in the sample and marking of a prozone-positive sample accordingly, and calculating from at least one of the signals measured during the reaction time the concentration of analyte in the sample by using a calibration curve or a portion of a calibration curve covering concentrations of analyte causing prozone effect for prozone positive samples, and another calibration curve or portion or calibration curve covering concentrations of analyte not causing prozone effect for the remaining samples.

    摘要翻译: 一种免疫测定方法,包括以下步骤:通过将样品与免疫反应物质混合来制备测量样品,所述样品能够特异性结合定义的分析物,允许样品在给定的反应时间内反应,测量与免疫活性物质的相互作用相关的信号 物质与样品中的分析物在反应时间之间,基于在不同时间测量的信号进行检查,相应地是否在样品中发生了前区效应,并且相应地标记了前带阳性样品,并且从 在反应期间测量的信号通过使用校准曲线或校准曲线的一部分覆盖分析物的浓度导致前区阳性样品的前区效应,以及另一个校准曲线或部分或校准曲线来覆盖样品中分析物的浓度 分析物对其余样品没有造成前区效应。