Stabilized reagent and method for determining creatine kinase
    2.
    发明授权
    Stabilized reagent and method for determining creatine kinase 有权
    稳定试剂和测定肌酸激酶的方法

    公开(公告)号:US06413733B1

    公开(公告)日:2002-07-02

    申请号:US09581357

    申请日:2000-06-09

    IPC分类号: C12Q150

    CPC分类号: C12Q1/50

    摘要: A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.

    摘要翻译: 一种用于通过从磷酸肌酸和ADP形成ATP并检测形成的ATP而测定生物样品材料中的肌酸激酶(CK)的稳定化试剂,所述ATP含有相对于添加的CK激活剂优选为亚摩尔量的有机或无机硫化合物 。 液体形式的相应试剂在2至8℃下稳定长达12个月,不会明显损失功能。

    Liquid reagent to detect creatine kinase
    4.
    发明授权
    Liquid reagent to detect creatine kinase 有权
    液体试剂检测肌酸激酶

    公开(公告)号:US06306617B1

    公开(公告)日:2001-10-23

    申请号:US09468416

    申请日:1999-12-20

    IPC分类号: C12Q150

    CPC分类号: C12Q1/50

    摘要: A liquid reagent for detecting creatine kinase activity. The liquid reagent includes G6PDH, hexokinase, creatine phosphate, glucose, ADP, NAD(P) and thioglycerol, which are split into two separate batches of reagents for storage. G6PDH is contained in one batch, and thioglycerol together with a further substance is contained in the other batch. The batches of reagents are mixed together prior to use for detection purposes.

    摘要翻译: 用于检测肌酸激酶活性的液体试剂。 液体试剂包括G6PDH,己糖激酶,磷酸肌酸,葡萄糖,ADP,NAD(P)和硫甘油,分成两批分开的试剂储存。 G6PDH包含在一批中,另一批含有硫甘油与另外的物质。 在使用前将批次的试剂混合在一起用于检测目的。