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公开(公告)号:US06599712B1
公开(公告)日:2003-07-29
申请号:US09869218
申请日:2001-07-24
IPC分类号: C12Q150
CPC分类号: C12Q1/008 , C12Q1/485 , G01N21/763
摘要: A method for measuring stains of a test sample by measuring adenosine phosphates. Luminescence of the adenosine phosphates is induced by a luminescent reagent (33) containing an ATP regenerating enzyme. The level or quantity of luminescence is detected by a silicon photodiode (5) to thereby measure the stains.
摘要翻译: 通过测量磷酸腺苷测量测试样品的污渍的方法。 通过含有ATP再生酶的发光试剂(33)诱导磷酸腺苷的发光。 通过硅光电二极管(5)检测发光的电平或数量,从而测量污渍。
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公开(公告)号:US06413733B1
公开(公告)日:2002-07-02
申请号:US09581357
申请日:2000-06-09
申请人: Rolf Nagel , Juergen Mistele , Norbert Schroeder
发明人: Rolf Nagel , Juergen Mistele , Norbert Schroeder
IPC分类号: C12Q150
CPC分类号: C12Q1/50
摘要: A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.
摘要翻译: 一种用于通过从磷酸肌酸和ADP形成ATP并检测形成的ATP而测定生物样品材料中的肌酸激酶(CK)的稳定化试剂,所述ATP含有相对于添加的CK激活剂优选为亚摩尔量的有机或无机硫化合物 。 液体形式的相应试剂在2至8℃下稳定长达12个月,不会明显损失功能。
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公开(公告)号:US06566086B1
公开(公告)日:2003-05-20
申请号:US09494205
申请日:2000-01-28
IPC分类号: C12Q150
CPC分类号: G01N33/70 , C07D471/22
摘要: Methods for the detection of creatine compound levels in body fluid samples are discussed. Portable kits capable of determining creatine levels using non-invasive and visually detectable methods are also included.
摘要翻译: 讨论了检测体液样品中肌酸化合物含量的方法。 还包括能够使用非侵入性和视觉上可检测的方法测定肌酸水平的便携式试剂盒。
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公开(公告)号:US06306617B1
公开(公告)日:2001-10-23
申请号:US09468416
申请日:1999-12-20
申请人: Gerhard Gunzer , Frank Kretzschmar , Katy Wrynn
发明人: Gerhard Gunzer , Frank Kretzschmar , Katy Wrynn
IPC分类号: C12Q150
CPC分类号: C12Q1/50
摘要: A liquid reagent for detecting creatine kinase activity. The liquid reagent includes G6PDH, hexokinase, creatine phosphate, glucose, ADP, NAD(P) and thioglycerol, which are split into two separate batches of reagents for storage. G6PDH is contained in one batch, and thioglycerol together with a further substance is contained in the other batch. The batches of reagents are mixed together prior to use for detection purposes.
摘要翻译: 用于检测肌酸激酶活性的液体试剂。 液体试剂包括G6PDH,己糖激酶,磷酸肌酸,葡萄糖,ADP,NAD(P)和硫甘油,分成两批分开的试剂储存。 G6PDH包含在一批中,另一批含有硫甘油与另外的物质。 在使用前将批次的试剂混合在一起用于检测目的。
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