公开(公告)号：US20050093417A1

公开(公告)日：2005-05-05

申请号：US10937918

申请日：2004-09-10

Applicant: Yogesh Gianchandani ,  Ghester Wilson ,  Long Que ,  Bhaskar Mitra ,  Ponnambalam Selvaganapathy

Inventor： Yogesh Gianchandani ,  Ghester Wilson ,  Long Que ,  Bhaskar Mitra ,  Ponnambalam Selvaganapathy

IPC: G01J3/30 ,  G01J3/443 ,  G01N21/64 ,  G01N21/67 ,  G01N21/69 ,  G02B20060101 ,  H01J13/00 ,  H01J17/00 ,  H01J61/58 ,  H01J61/64

CPC classification number: H01J61/58 ,  G01N21/645 ,  G01N21/67 ,  G01N21/69 ,  G01N2021/6417

Abstract: A micro-discharge optical source apparatus and a method and system which utilizes the apparatus to analyze a sample are provided. The apparatus includes a substrate and an anode electrode and a cathode electrode supported on the substrate. One of the electrodes, such as the cathode, is preferably a liquid electrode. The electrodes are spaced apart to define an inter-electrode gap therebetween. The apparatus further includes a voltage source for applying a voltage between the electrodes sufficient to generate a glow micro-discharge in the gap to cause a first emission having a first desired excitation spectrum. An optical element is integrated with the substrate to direct the first emission to travel along an optical path including an axis. In one embodiment, the system detects the direct fluorescence of a sample such as tryptophan and other amino acids. In another embodiment, the system detects fluorescence of dye-labeled biochemicals.

Abstract translation: 提供一种微放电光源装置以及利用该装置分析样品的方法和系统。 该装置包括基板和支撑在基板上的阳极电极和阴极电极。 诸如阴极的电极之一优选为液体电极。 电极间隔开以限定它们之间的电极间间隙。 该装置还包括用于在电极之间施加足以在间隙中产生辉光微放电以产生具有第一期望激发光谱的第一发射的电压的电压源。 光学元件与衬底集成以引导第一发射沿着包括轴的光路行进。 在一个实施方案中，系统检测样品如色氨酸和其它氨基酸的直接荧光。 在另一个实施方案中，该系统检测染料标记的生物化学物质的荧光。