公开(公告)号：US09040302B2

公开(公告)日：2015-05-26

申请号：US13697860

申请日：2011-05-19

申请人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

发明人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

IPC分类号： A61K35/74 ,  A61K38/16 ,  A23C9/123 ,  A23L1/30 ,  C12N1/06 ,  C12P21/02 ,  A61K35/00

CPC分类号： A61K38/164 ,  A23C9/1238 ,  A23L33/135 ,  A61K35/74 ,  A61K2035/11 ,  C12N1/06 ,  C12P21/02

摘要： Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.

摘要翻译： 提供用于靶向递送生物治疗剂的方法和组合物。 组合物包含胆汁敏感的嗜热链球菌，其被修饰以在胆汁暴露后释放生物治疗剂。 由本文公开的嗜热链球菌细菌释放的生物治疗剂包括AQ和AQR丰富的肽。 本发明的方法包括向受试者施用在胆汁暴露后经修饰以释放生物治疗剂的嗜热链球菌。 圣氏嗜热链霉菌的施用促进期望的治疗反应。 可以修饰细菌以表达和释放富含AQ或AQR的肽，其随后抑制细胞凋亡或减少粘膜损伤。 因此，本发明的方法用于治疗或预防各种胃肠疾病，包括艰难梭菌感染和抗生素相关性腹泻。

公开(公告)号：US20130259834A1

公开(公告)日：2013-10-03

申请号：US13697860

申请日：2011-05-19

申请人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

发明人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

IPC分类号： A61K38/16 ,  A61K35/74

CPC分类号： A61K38/164 ,  A23C9/1238 ,  A23L33/135 ,  A61K35/74 ,  A61K2035/11 ,  C12N1/06 ,  C12P21/02

摘要： Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.

摘要翻译： 提供了用于靶向递送生物治疗剂的方法和组合物。 组合物包含胆汁敏感的嗜热链球菌，其被修饰以在胆汁暴露后释放生物治疗剂。 由本文公开的嗜热链球菌细菌释放的生物治疗剂包括AQ和AQR丰富的肽。 本发明的方法包括向受试者施用在胆汁暴露后经修饰以释放生物治疗剂的嗜热链球菌。 圣氏嗜热链霉菌的施用促进期望的治疗反应。 可以修饰细菌以表达和释放富含AQ或AQR的肽，其随后抑制细胞凋亡或减少粘膜损伤。 因此，本发明的方法用于治疗或预防各种胃肠疾病，包括艰难梭菌感染和抗生素相关性腹泻。