公开(公告)号：US20060090218A1

公开(公告)日：2006-04-27

申请号：US11301924

申请日：2005-12-13

申请人： Joshua Levin ,  Kenneth Phillips ,  Gregory Budziszewski ,  Frederick Meins ,  Evgueni Glazov

发明人： Joshua Levin ,  Kenneth Phillips ,  Gregory Budziszewski ,  Frederick Meins ,  Evgueni Glazov

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8218

摘要： The present invention relates to methods to regulate gene expression in plants. In particular, manipulation of the expression in a plant cell of a nucleotide sequence encoding a polypeptide comprising a 3′-5′ exonuclease domain is disclosed. More stable and predictable expression is thus obtained. The present invention also relates to method of increasing or decreasing post-transcriptional silencing. The invention further relates to novel nucleic acid molecules comprising nucleotide sequences encoding polypeptides comprising a 3′-5′ exonuclease domain.

摘要翻译： 本发明涉及调节植物中基因表达的方法。 特别地，公开了编码包含3'-5'外切核酸酶结构域的多肽的核苷酸序列在植物细胞中的表达的操作。 从而获得更稳定和可预测的表达。 本发明还涉及增加或减少转录后沉默的方法。 本发明还涉及包含编码包含3'-5'外切核酸酶结构域的多肽的核苷酸序列的新型核酸分子。

公开(公告)号：US20070250946A1

公开(公告)日：2007-10-25

申请号：US11659129

申请日：2005-08-04

申请人： Robert Ascenzi ,  Gregory Budziszewski ,  Genichi Kakefuda ,  Bijay Singh

发明人： Robert Ascenzi ,  Gregory Budziszewski ,  Genichi Kakefuda ,  Bijay Singh

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01N43/50 ,  C07H21/04 ,  C07K1/00 ,  C12N15/00 ,  C12N5/00

CPC分类号： C12Y202/01006 ,  C12N9/88 ,  C12N15/8274 ,  C12N15/8278

摘要： Isolated polynucleotides that encode acetohydroxyacid synthase small subunit (AHASS) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and expression vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, expression cassettes, and expression vectors, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides are also described.

摘要翻译： 描述了编码乙酰羟酸合酶小亚基（AHASS）多肽的分离的多核苷酸，以及编码这些多肽的氨基酸序列。 描述了包含本发明的多核苷酸的表达盒和表达载体，以及用多核苷酸，表达盒和表达载体转化的植物和宿主细胞。 还描述了使用多核苷酸增强植物对除草剂的抗性的方法。