公开(公告)号：US20080008993A1

公开(公告)日：2008-01-10

申请号：US10556030

申请日：2003-05-09

申请人： Guenter Kahl ,  Peter Winter ,  Detlev Krueger ,  Stefanie Reich ,  Hideo Matsumura ,  Ryohei Terauchi

发明人： Guenter Kahl ,  Peter Winter ,  Detlev Krueger ,  Stefanie Reich ,  Hideo Matsumura ,  Ryohei Terauchi

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/00

CPC分类号： C12Q1/6809 ,  C12Q1/6837 ,  C12Q2539/103 ,  C12Q2525/204 ,  C12Q2521/301 ,  C12Q2545/114

摘要： The type III restriction enzyme EcoP15I is used to isolate from cDNA of an expressed gene a tag comprising more than 25 nucleotides and capable of identifying the expressed gene, wherein the 3′ end of the tag is defined by a cleavage site of the type III restriction enzyme and the 5′ end of the tag is defined by the cleavage site of another restriction enzyme that is closest to the 3′ end of the cDNA of the expressed gene. The tag of the invention allows accurate quantitative gene expression analysis and rapid gene expression profiling in any organism for which no expressed sequence tag (EST) database is available.

摘要翻译： III型限制酶EcoP15I用于从表达基因的cDNA分离包含多于25个核苷酸并且能够鉴定表达基因的标签，其中标签的3'末端由III型限制酶切割位点 酶和标签的5'末端由最接近表达基因的cDNA的3'末端的另一限制酶的切割位点定义。 本发明的标签允许在没有表达序列标签（EST）数据库的任何生物体中进行准确的定量基因表达分析和快速的基因表达谱。