公开(公告)号：US5714336A

公开(公告)日：1998-02-03

申请号：US884874

申请日：1992-05-18

Applicant: Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

Inventor： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

IPC: G01N33/53 ,  G01N33/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/78

CPC classification number: G01N33/536 ,  G01N33/0055 ,  G01N33/78

Abstract: A method for determining the concentration of the free fraction of an active compound, present in a biological fluid, in the presence of natural binders, the free and bound fractions of the active compound being in mutual equilibrium, by a) contacting a sample of the fluid with an unlabeled antibody, b) separating the sample from the unlabeled antibody, c) incubating the unlabeled antibody with a labeled substance (tracer) for cross-reaction with the antibody and d) measuring the amount of the tracer which is or is not bound to the antibody and calculating from this the concentration of the free fraction of the active compound, wherein the quantity of the unlabeled antibody and/or its affinity for the active compound are so small that they do not substantially effect the equilibrium between the free and bound fractions of the active compound, and the affinity of the tracer for the antibody is substantially higher or substantially lower than that of the active compound itself, and a test kit suitable for this method.

公开(公告)号：US06103486A

公开(公告)日：2000-08-15

申请号：US75832

申请日：1993-06-14

Applicant: Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

Inventor： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

IPC: G01N33/53 ,  G01N33/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/78

CPC classification number: G01N33/536 ,  G01N33/0055 ,  G01N33/78

Abstract: A method for determining the concentration of the free fraction of an active compound, present in a biological fluid, in the presence of natural binders, the free and bound fractions of the active compound being in mutual equilibrium, bya) contacting a sample of the fluid with an unlabeled antibody,b) separating the sample from the unlabeled antibody,c) incubating the unlabeled antibody with a labeled substance (tracer) for cross-reaction with the antibody andd) measuring the amount of the tracer which is or is not bound to the antibody and calculating from this the concentration of the free fraction of the active compound,wherein the quantity of the unlabeled antibody and/or its affinity for the active compound are so small that they do not substantially effect the equilibrium between the free and bound fractions of the active compound, and the affinity of the tracer for the antibody is substantially higher or substantially lower than that of the active compound itself, and a test kit suitable for this method.

Abstract translation: 在天然粘合剂存在下，确定存在于生物流体中的活性化合物的游离级分浓度的方法，所述活性化合物的游离和结合级分是相互平衡的，所述方法是通过以下步骤： 具有未标记抗体的液体，b）从未标记的抗体中分离样品，c）将未标记的抗体与标记物质（示踪剂）孵育以与抗体交叉反应，以及d）测量示踪剂的量或不是 与抗体结合，从此计算活性化合物的游离级分的浓度，其中未标记的抗体的量和/或其对活性化合物的亲和力如此之小，使得它们基本上不影响游离和 活性化合物的结合部分，示踪剂对抗体的亲和力显着高于或显着低于活性化合物本身的亲和力， t kit适用于此方法。

公开(公告)号：US4820860A

公开(公告)日：1989-04-11

申请号：US87805

申请日：1987-08-21

Applicant: Hans Wissmann ,  Guido Simons ,  Helmut Strecker, deceased

Inventor： Hans Wissmann ,  Guido Simons ,  Helmut Strecker, deceased

IPC: C08G65/08 ,  C07C67/00 ,  C07C239/00 ,  C07C271/22 ,  C08G65/02 ,  C08G65/28 ,  C08G65/32 ,  C08G65/333 ,  C08G65/48 ,  G01N33/53 ,  G01N33/78 ,  C07C101/32

CPC classification number: C08G65/33396 ,  G01N33/78

Abstract: The invention relates to novel thyronine derivatives of the formula ##STR1## in which n is 10-400, R denotes H, alkyl or N-carbonylthyronine, R.sup.1 and R.sup.2 are identical or different and denote hydrogen or iodine, and R.sup.3 denotes H or alkyl, a process for the preparation of the compounds, and their use when carrying out immunoassays.

Abstract translation: 本发明涉及其中n为10-400，R表示H，烷基或N-羰基甲状腺氨酸的式（I）＆lt; IMAGE＆gt;的新颖的甲状腺素衍生物，R 1和R 2相同或不同，表示氢或碘， 并且R3表示H或烷基，制备化合物的方法及其在进行免疫测定时的用途。

公开(公告)号：US5126242A

公开(公告)日：1992-06-30

申请号：US536809

申请日：1990-06-12

Applicant: Henning Hachmann ,  Peter Molz ,  Stephan Neuenhofer ,  Gerd Schnorr ,  Guido Simons ,  Heinz-Jurgen Skrzipczyk ,  Kurt E. Weimer

Inventor： Henning Hachmann ,  Peter Molz ,  Stephan Neuenhofer ,  Gerd Schnorr ,  Guido Simons ,  Heinz-Jurgen Skrzipczyk ,  Kurt E. Weimer

IPC: G01N33/543

CPC classification number: G01N33/54393 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S530/815 ,  Y10T428/2998

Abstract: The invention relates to a process for the stabilization of biologically active substances which are immobilized on a carrier, where the solid phase with the immobilized biologically active substance is contacted for the stabilization with a solution which contains polyanetholesulfonic acid and/or salts thereof. The biologically active substances are, in particular, antibodies.

Abstract translation: 本发明涉及一种固定在载体上的生物活性物质稳定化方法，其中固定的生物活性物质的固相与包含聚苯醚磺酸和/或其盐的溶液进行稳定化。 生物活性物质尤其是抗体。

公开(公告)号：US4814484A

公开(公告)日：1989-03-21

申请号：US1207

申请日：1987-01-07

Applicant: Hans Wissmann ,  Henning Hachmann ,  Guido Simons ,  Helmut Strecker

Inventor： Hans Wissmann ,  Henning Hachmann ,  Guido Simons ,  Helmut Strecker

IPC: G01N33/53 ,  C07B31/00 ,  C07B59/00 ,  C07C67/00 ,  C07F9/30 ,  C07F9/38 ,  C07F9/40 ,  G01N33/78 ,  C07C101/30

CPC classification number: G01N33/78 ,  C07F9/301 ,  C07F9/3808

Abstract: The invention relates to new thyronine derivatives of the formula ##STR1## in which n is 1-6, R denotes hydroxyl, alkyl or aryl, R.sup.1 and R.sup.2 are identical or different and denote iodine or hydrogen, and R.sup.3 denotes hydrogen, alkyl or aralkyl, a process for the preparation thereof, and the use thereof for carrying out radioimmunoassays.

Abstract translation: 本发明涉及式（I）的新的甲状腺衍生物，其中n为1-6，R表示羟基，烷基或芳基，R 1和R 2相同或不同，表示碘或氢，R3表示氢， 烷基或芳烷基，其制备方法及其用于进行放射免疫测定的用途。