公开(公告)号：US5126242A

公开(公告)日：1992-06-30

申请号：US536809

申请日：1990-06-12

申请人： Henning Hachmann ,  Peter Molz ,  Stephan Neuenhofer ,  Gerd Schnorr ,  Guido Simons ,  Heinz-Jurgen Skrzipczyk ,  Kurt E. Weimer

发明人： Henning Hachmann ,  Peter Molz ,  Stephan Neuenhofer ,  Gerd Schnorr ,  Guido Simons ,  Heinz-Jurgen Skrzipczyk ,  Kurt E. Weimer

IPC分类号： G01N33/543

CPC分类号： G01N33/54393 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S530/815 ,  Y10T428/2998

摘要： The invention relates to a process for the stabilization of biologically active substances which are immobilized on a carrier, where the solid phase with the immobilized biologically active substance is contacted for the stabilization with a solution which contains polyanetholesulfonic acid and/or salts thereof. The biologically active substances are, in particular, antibodies.

摘要翻译： 本发明涉及一种固定在载体上的生物活性物质稳定化方法，其中固定的生物活性物质的固相与包含聚苯醚磺酸和/或其盐的溶液进行稳定化。 生物活性物质尤其是抗体。

公开(公告)号：US06103486A

公开(公告)日：2000-08-15

申请号：US75832

申请日：1993-06-14

申请人： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

发明人： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

IPC分类号： G01N33/53 ,  G01N33/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/78

CPC分类号： G01N33/536 ,  G01N33/0055 ,  G01N33/78

摘要： A method for determining the concentration of the free fraction of an active compound, present in a biological fluid, in the presence of natural binders, the free and bound fractions of the active compound being in mutual equilibrium, bya) contacting a sample of the fluid with an unlabeled antibody,b) separating the sample from the unlabeled antibody,c) incubating the unlabeled antibody with a labeled substance (tracer) for cross-reaction with the antibody andd) measuring the amount of the tracer which is or is not bound to the antibody and calculating from this the concentration of the free fraction of the active compound,wherein the quantity of the unlabeled antibody and/or its affinity for the active compound are so small that they do not substantially effect the equilibrium between the free and bound fractions of the active compound, and the affinity of the tracer for the antibody is substantially higher or substantially lower than that of the active compound itself, and a test kit suitable for this method.

摘要翻译： 在天然粘合剂存在下，确定存在于生物流体中的活性化合物的游离级分浓度的方法，所述活性化合物的游离和结合级分是相互平衡的，所述方法是通过以下步骤： 具有未标记抗体的液体，b）从未标记的抗体中分离样品，c）将未标记的抗体与标记物质（示踪剂）孵育以与抗体交叉反应，以及d）测量示踪剂的量或不是 与抗体结合，从此计算活性化合物的游离级分的浓度，其中未标记的抗体的量和/或其对活性化合物的亲和力如此之小，使得它们基本上不影响游离和 活性化合物的结合部分，示踪剂对抗体的亲和力显着高于或显着低于活性化合物本身的亲和力， t kit适用于此方法。

公开(公告)号：US5879953A

公开(公告)日：1999-03-09

申请号：US479196

申请日：1995-06-07

申请人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

发明人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

IPC分类号： C09B15/00 ,  G01N33/533 ,  G01N33/58 ,  G01N33/543

CPC分类号： G01N33/533 ,  C09B15/00 ,  G01N33/582 ,  Y10S436/80

摘要： Chemiluminescent acridinium derivatives of the formula I ##STR1## in which R.sup.4 represents a radical of the formula II or III ##STR2## and A.sup..theta., X--, R.sup.1 -R.sup.3, R.sup.5 and R.sup.6 have the stated meanings, as well as processes for the preparation of the compounds of the formula (I) and the use thereof in chemiluminescence immunoassays.

摘要翻译： （I）的化学发光吖啶鎓衍生物，其中R 4表示式II或III基团（III）和A theta，X-，R1-R3，R5和 R6具有所述含义，以及制备式（I）化合物的方法及其在化学发光免疫测定中的用途。

公开(公告)号：US5783696A

公开(公告)日：1998-07-21

申请号：US474552

申请日：1995-06-07

申请人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

发明人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

IPC分类号： C09B15/00 ,  G01N33/533 ,  G01N33/58

CPC分类号： G01N33/533 ,  C09B15/00 ,  G01N33/582 ,  Y10S436/80

摘要： Chemiluminescent acridinium derivatives of the formula I ##STR1## in which R.sup.4 represents a radical of the formula II or III ##STR2## and A.sup..theta., X--, R.sup.1 -R.sup.3, R.sup.5 and R.sup.6 have the stated meanings, as well as processes for the preparation of the compounds of the formula (I) and the use thereof in chemiluminescence immunoassays.

摘要翻译： （I）的化学发光吖啶鎓衍生物，其中R 4表示式II或III基团（III）和A theta，X-，R1-R3，R5和 R6具有所述含义，以及制备式（I）化合物的方法及其在化学发光免疫测定中的用途。

公开(公告)号：US6010868A

公开(公告)日：2000-01-04

申请号：US750743

申请日：1996-12-20

申请人： Stephan Neuenhofer ,  Reinhard Kasmarker ,  Gotz Walter ,  Hans-Peter Harthus ,  Gunther Nau ,  Heinz-Jurgen Skrzipczyk ,  Peter Molz ,  Norbert Madry

发明人： Stephan Neuenhofer ,  Reinhard Kasmarker ,  Gotz Walter ,  Hans-Peter Harthus ,  Gunther Nau ,  Heinz-Jurgen Skrzipczyk ,  Peter Molz ,  Norbert Madry

IPC分类号： G01N33/531 ,  C12N9/96 ,  G01N33/532 ,  G01N33/577 ,  G01N33/58 ,  G01N33/53

CPC分类号： G01N33/582 ,  C12N9/96 ,  G01N33/532 ,  G01N33/58 ,  Y10S435/963 ,  Y10S435/975

摘要： The invention concerns a method of stabilizing molecules, or parts of molecules, which are sensitive to hydrolysis, in particular hydrolysis-sensitive labels or label-containing tracers in aqueous solutions. The invention also concerns kits for carrying out immunological assays making use of the method proposed.

摘要翻译： PCT No.PCT / EP95 / 02446 Sec。 371日期1996年12月20日第 102（e）日期1996年12月20日PCT提交1995年6月23日PCT公布。 出版物WO96 / 00284 日期1996年1月4日本发明涉及稳定对水解敏感的分子或部分分子的方法，特别是在水溶液中水解敏感的标记或标记的示踪剂。 本发明还涉及使用所提出的方法进行免疫测定的试剂盒。

公开(公告)号：US6002007A

公开(公告)日：1999-12-14

申请号：US93694

申请日：1993-07-20

申请人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

发明人： Tonio Kinkel ,  Peter Molz ,  Erwin Schmidt ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk

IPC分类号： C09B15/00 ,  G01N33/533 ,  G01N33/58 ,  C07D219/10

CPC分类号： G01N33/533 ,  C09B15/00 ,  G01N33/582 ,  Y10S436/80

摘要： Chemiluminescent acridinium derivatives of the formula I ##STR1## in which R.sup.4 represents a radical of the formula II or III ##STR2## and A.sup..theta., X--, R.sup.1 -R.sup.3, R.sup.5 and R.sup.6 have the stated meanings, as well as processes for the preparation of the compounds of the formula (I) and the use thereof in chemiluminescence immunoassays.

摘要翻译： 其中R 4表示式II或III的基团和A theta，X-，R 1 -R 3，R 5和R 6的化学发光吖啶鎓衍生物具有所述含义，以及制备式 式（I）化合物及其在化学发光免疫测定中的应用。

公开(公告)号：US5714336A

公开(公告)日：1998-02-03

申请号：US884874

申请日：1992-05-18

申请人： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

发明人： Guido Simons ,  Helmut Strecker, deceased ,  Peter Molz ,  Gerd Schnorr ,  Heinz Jurgen Skrzipczyk ,  Hans Wissmann

IPC分类号： G01N33/53 ,  G01N33/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/78

CPC分类号： G01N33/536 ,  G01N33/0055 ,  G01N33/78

摘要： A method for determining the concentration of the free fraction of an active compound, present in a biological fluid, in the presence of natural binders, the free and bound fractions of the active compound being in mutual equilibrium, by a) contacting a sample of the fluid with an unlabeled antibody, b) separating the sample from the unlabeled antibody, c) incubating the unlabeled antibody with a labeled substance (tracer) for cross-reaction with the antibody and d) measuring the amount of the tracer which is or is not bound to the antibody and calculating from this the concentration of the free fraction of the active compound, wherein the quantity of the unlabeled antibody and/or its affinity for the active compound are so small that they do not substantially effect the equilibrium between the free and bound fractions of the active compound, and the affinity of the tracer for the antibody is substantially higher or substantially lower than that of the active compound itself, and a test kit suitable for this method.