公开(公告)号：US20210324391A1

公开(公告)日：2021-10-21

申请号：US17271356

申请日：2019-05-31

申请人： ZHEJIANG NHU COMPANY LTD. ,  HEILONGJIANG NHU BIOTECHNOLOGY COMPANY TLD. ,  SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD. ,  ZHEJIANG UNIVERSITY

发明人： Hongwei Yu ,  Shenfeng YUAN ,  Yongqiang ZHU ,  Kai YU ,  Zhirong CHEN ,  Yong LI ,  Guisheng QIU ,  Xiaoqing LIU

IPC分类号： C12N15/74 ,  C07K14/245 ,  C12P7/66 ,  C12N1/20

摘要： A recombinant microorganism, a preparation method thereof and its application in the production of coenzyme Q10. Specifically, the present disclosure provides a method of exogenously introducing a gene encoding the global regulatory protein irrE to construct a recombinant microorganism. This recombinant microorganism is suitable for producing coenzyme Q10 by fermentation method, and is particularly suitable for producing oxidized coenzyme Q10. The recombinant microorganism of the present disclosure has stress resistance, and has good tolerance against harsh environments including high osmotic pressure and high redox potential, thus making it possible to significantly increase the yield of coenzyme Q10, especially the yield of oxidized coenzyme Q10.