公开(公告)号：US20210324391A1

公开(公告)日：2021-10-21

申请号：US17271356

申请日：2019-05-31

申请人： ZHEJIANG NHU COMPANY LTD. ,  HEILONGJIANG NHU BIOTECHNOLOGY COMPANY TLD. ,  SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD. ,  ZHEJIANG UNIVERSITY

发明人： Hongwei Yu ,  Shenfeng YUAN ,  Yongqiang ZHU ,  Kai YU ,  Zhirong CHEN ,  Yong LI ,  Guisheng QIU ,  Xiaoqing LIU

IPC分类号： C12N15/74 ,  C07K14/245 ,  C12P7/66 ,  C12N1/20

摘要： A recombinant microorganism, a preparation method thereof and its application in the production of coenzyme Q10. Specifically, the present disclosure provides a method of exogenously introducing a gene encoding the global regulatory protein irrE to construct a recombinant microorganism. This recombinant microorganism is suitable for producing coenzyme Q10 by fermentation method, and is particularly suitable for producing oxidized coenzyme Q10. The recombinant microorganism of the present disclosure has stress resistance, and has good tolerance against harsh environments including high osmotic pressure and high redox potential, thus making it possible to significantly increase the yield of coenzyme Q10, especially the yield of oxidized coenzyme Q10.

公开(公告)号：US10982242B2

公开(公告)日：2021-04-20

申请号：US15757936

申请日：2016-10-21

申请人： SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD. ,  ZHEJIANG UNIVERSITY ,  ZHEJIANG NHU COMPANY LTD.

发明人： Hongwei Yu ,  Yong Lv ,  Yong Li ,  Jianbo Chen ,  Shaofeng Chen ,  Weifeng Li ,  Hongmei Zhang ,  Yongqiang Zhu

IPC分类号： C12P7/66 ,  C12Q3/00 ,  C12N1/20 ,  C12R1/01

摘要： The present invention relates to an optimized fermentation process of coenzyme Q10, particularly to a fermentation process of coenzyme Q10 via flow feeding based on cooperative control of changes of online oxygen consumption rate and conductivity. During the fermentation process of coenzyme Q10 production strains, the oxygen consumption rate is controlled between 30-150 mmol/L·h and the conductivity is maintained between 3.0-30.0 ms/cm via flow feeding, so as to facilitate strain growth and the start of coenzyme Q10 synthesis and accumulation. The present invention can substantially increase output of coenzyme Q10 and greatly reduce the production cost with simple process control and strong operability, thus being applicable to large-scale industrial production.

公开(公告)号：US10774350B2

公开(公告)日：2020-09-15

申请号：US16016813

申请日：2018-06-25

申请人： ZHEJIANG NHU COMPANY LTD. ,  ZHEJIANG UNIVERSITY ,  HEILONGJIANG NHU BIOTECHNOLOGY COMPANY LTD. ,  SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD.

发明人： Shenfeng Yuan ,  Hongwei Yu ,  Zhaofeng Chen ,  Yongqiang Zhu ,  Yi Min ,  Yong Li ,  Baishan Hu ,  Guisheng Qiu ,  Kai Yu

IPC分类号： C12P7/66

摘要： The present application relates to a method for fermentative production of oxidized coenzyme Q10 and high-content oxidized coenzyme Q10 prepared therefrom. For the method for fermentative production of oxidized coenzyme Q10, in a fermentation process of a production strain, the oxidation-reduction potential (ORP) of a fermentation broth is controlled to be −50 to 300 Mv, and preferably the oxidation-reduction potential (ORP) of the fermentation broth is controlled to be 50 to 200 mV. By controlling the ORP of the fermentation broth, the method for fermentative production of oxidized coenzyme Q10 enables the oxidized coenzyme Q10 content in the coenzyme Q10 produced by microorganisms to reach 96% or more, and the product is substantially composed of a single component, which makes post-treatment more convenient. Oxidized coenzyme Q10 is more stable than reduced coenzyme Q10, and as compared with the coenzyme Q10 obtained by fermentative production in the prior art, high-content oxidized coenzyme Q10 degrades in a less amount in organisms. In addition, the fermentation method of the present application has a high potency.