公开(公告)号：US06638743B2

公开(公告)日：2003-10-28

申请号：US10119354

申请日：2002-04-09

申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jörg Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jörg Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

IPC分类号： C12N1300

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

摘要： A method is provided for measuring a state variable of a biological cell (3) located in a nutrient medium (2) and supported on and adhering to a support area (5). Within the support area (5) for the cell (3) and at a distance from the support area edge, an opening is made in the membrane of the cell (3). The edge of the cell membrane that surrounds the opening and adheres to the support area (5) seals off the liquid found inside the cell (3) from the nutrient medium (2). Through the opening the state variable (2) is measured. An apparatus for performing the method is also provided.