公开(公告)号：US20100251438A1

公开(公告)日：2010-09-30

申请号：US12725013

申请日：2010-03-16

申请人： Heinrich Huber ,  Heiko Dussmann ,  Paul Perrine ,  Jakub Wenus ,  Dimitrios Kalamatianos ,  Maximilian Wuerstle

发明人： Heinrich Huber ,  Heiko Dussmann ,  Paul Perrine ,  Jakub Wenus ,  Dimitrios Kalamatianos ,  Maximilian Wuerstle

IPC分类号： G01Q10/02

CPC分类号： G02B21/365 ,  G01N15/1475 ,  G02B21/008

摘要： A method for controlling laser scanning microscopy of a probe comprising at least one cell is disclosed. The method comprises the steps of acquiring at least one initial image of the probe and identifying at least one cell within an initial probe image. Using a pre-defined grammar, a first set of scanning mode parameters for monitoring the cell(s); a first set of trigger parameters including at least one physiological parameter defining an event in the cell(s); and a second set of scanning mode parameters for monitoring at least one cell of the probe after an occurrence of the event is defined. A successive set of probe images acquired according to the first set of scanning mode parameters is provided and processed to determine if the event has occurred. Responsive to the event occurring, microscope modality is changed to the second set of scanning mode parameters.

摘要翻译： 公开了一种用于控制包括至少一个电池的探针的激光扫描显微镜的方法。 该方法包括获取探针的至少一个初始图像并识别初始探针图像内的至少一个单元的步骤。 使用预定义的语法，用于监视单元的第一组扫描模式参数; 包括定义细胞中的事件的至少一个生理参数的第一组触发参数; 以及第二组扫描模式参数，用于在发生事件之后监视探测器的至少一个单元。 提供并处理根据第一组扫描模式参数获取的连续的探测图像组，以确定事件是否已经发生。 响应于发生的事件，显微镜模态改为第二组扫描模式参数。