公开(公告)号：US11972620B2

公开(公告)日：2024-04-30

申请号：US17076008

申请日：2020-10-21

Applicant: Cytek Biosciences, Inc.

Inventor： Alan Li ,  Shobana Vaidyanathan

IPC: G06V20/69 ,  G01N15/14 ,  G06T7/00 ,  G06T7/11

CPC classification number: G06V20/698 ,  G01N15/1475 ,  G06T7/0012 ,  G06T7/11 ,  G06V20/693 ,  G06V20/695 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06V2201/03

Abstract: A classifier engine provides cell morphology identification and cell classification in computer-automated systems, methods and diagnostic tools. The classifier engine performs multispectral segmentation of thousands of cellular images acquired by a multispectral imaging flow cytometer. As a function of imaging mode, different ones of the images provide different segmentation masks for cells and subcellular parts. Using the segmentation masks, the classifier engine iteratively optimizes model fitting of different cellular parts. The resulting improved image data has increased accuracy of location of cell parts in an image and enables detection of complex cell morphologies in the image. The classifier engine provides automated ranking and selection of most discriminative shape based features for classifying cell types.

公开(公告)号：US20240085399A1

公开(公告)日：2024-03-14

申请号：US18508899

申请日：2023-11-14

Applicant: Leuko Labs, Inc.

Inventor： Carlos Castro Gonzalez ,  Ian Butterworth ,  Aurelien Bourquard ,  Alvaro Sanchez Ferro

IPC: G01N33/49 ,  G01N15/14 ,  G01N21/31 ,  G06F18/214 ,  G06T7/00 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/69 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70

CPC classification number: G01N33/49 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/31 ,  G06F18/2148 ,  G06T7/0016 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/695 ,  G06V20/698 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N2015/0073 ,  G06T2207/10016 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30101 ,  G06T2207/30242

Abstract: In one aspect, a method to detect white blood cells and/or white blood cell subtypes from non-invasive capillary videos is featured. The method includes acquiring a first plurality of images of a region of interest including one or more capillaries of a predetermined area of a human subject from non-invasive capillary videos captured with an optical device, processing the first plurality of images to determine one or more optical absorption gaps located in said capillary, and annotating the first plurality of images with an indication of any optical absorption gap detected in the first plurality of images. The method also includes acquiring a second plurality of images of the same region of interest of the same capillary with an advanced optical device capable of resolving cellular structure of white blood cells and white blood cell subtypes and spatiotemporally annotating the second plurality of images with an indication of any white blood cell detected and/or a subtype of any white blood cell detected in the second plurality of images. The method also includes inputting the first plurality of images and annotated information from the first plurality of images and annotated information from the spatiotemporally annotated second plurality of images into a machine learning subsystem configured to determine a presence of white blood cells and/or the subtype of any white blood cells present in the one or more optical absorption gaps in the first plurality of images.

公开(公告)号：US11913029B2

公开(公告)日：2024-02-27

申请号：US18061811

申请日：2022-12-05

Applicant: Cellino Biotech, Inc.

Inventor： Matthias Wagner ,  Suvi Aivio ,  Mariangela Amenduni ,  Catherine Pilsmaker ,  Arnaldo Pereira ,  Ananya Zutshi ,  Anthia Toure ,  Steven Nagle ,  Ozge Whiting ,  George Harb ,  Matthew Sullivan ,  Maya Berlin-Udi ,  Stefanie Morgan ,  Nick Seay ,  Sang Lee ,  Scott Luro

IPC: G06T7/00 ,  B01L3/00 ,  B01L9/00 ,  C12M1/00 ,  C12M1/06 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00 ,  C12N5/00 ,  C12N5/074 ,  G01N15/10 ,  G01N15/14

CPC classification number: C12N5/0696 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/52 ,  C12M23/14 ,  C12M23/22 ,  C12M23/26 ,  C12M23/28 ,  C12M23/42 ,  C12M23/44 ,  C12M23/48 ,  C12M25/06 ,  C12M27/02 ,  C12M29/02 ,  C12M31/02 ,  C12M33/00 ,  C12M33/12 ,  C12M41/12 ,  C12M41/26 ,  C12M41/44 ,  C12M41/46 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0081 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G06T7/0012 ,  G06T7/0016 ,  B01L2200/18 ,  B01L2300/0663 ,  B01L2300/0877 ,  C12M41/36 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1454 ,  G01N2015/1486 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

公开(公告)号：US20240044767A1

公开(公告)日：2024-02-08

申请号：US18484297

申请日：2023-10-10

Applicant: Honeywell International Inc.

Inventor： Adam Dewey MCBRADY ,  Stephan Michael BORK

IPC: G01N15/06 ,  G01N15/08 ,  G01N15/14 ,  G01N33/00 ,  G01N15/02

CPC classification number: G01N15/0606 ,  G01N15/082 ,  G01N15/1475 ,  G01N33/0004 ,  G01N15/0227 ,  G01N15/0618 ,  G01N15/0625 ,  G01N2015/1402

Abstract: Various embodiments described herein relate to apparatuses and methods for detecting fluid particles and their characteristics. In various embodiments, a device for detecting fluid particles and their characteristics may comprise a fluid composition sensor configured to receive a volume of fluid. The fluid composition sensor has a collection media housing configured to receive a portion of a collection media, a pump for moving a volume of fluid over the collection media housing, an imaging device configured to capture an image of particles on the collection media, and a particle matter mass concentration calculation circuitry configured to calculate a total particle matter mass. The particle matter mass concentration calculation circuitry is connected with the imaging device and the pump. The particle matter mass concentration calculation circuitry is configured to adjust the volume of fluid over the collection media housing.

公开(公告)号：US11892390B2

公开(公告)日：2024-02-06

申请号：US16858399

申请日：2020-04-24

Applicant: NEW YORK UNIVERSITY

Inventor： David G. Grier ,  Fook Chiong Cheong ,  Ke Xiao

IPC: G01N15/14 ,  G02B21/00 ,  G03H1/00 ,  G01N15/00

CPC classification number: G01N15/1475 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G02B21/008 ,  G02B21/0056 ,  G02B21/0068 ,  G03H1/0005 ,  G01N15/1484 ,  G01N2015/003 ,  G01N2015/0065 ,  G01N2015/1445 ,  G01N2015/1454 ,  G01N2015/1493 ,  G01N2015/1497 ,  G03H2001/005

Abstract: An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

公开(公告)号：US20240035953A1

公开(公告)日：2024-02-01

申请号：US18486428

申请日：2023-10-13

Applicant: Teracyte Ltd.

Inventor： Gal LENZ ,  Yoav NISSAN-COHEN

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1475 ,  G01N21/6428 ,  G01N2021/6439

Abstract: Improved methods for analyzing cells by combining information from the same cell before and after lysis. The methods of the present invention allow examination of the interactions or relationships between cellular physiological parameter that can only be measured in living cells (i.e., metabolic state) and the expression of proteins and peptides of interest. Single cell processing is made possible by determination of wells containing single cells therein and avoiding analysis of well containing no or multiple cells therein. Fluorescent and/or bright-field imaging may be used in certain embodiments.

公开(公告)号：US20240027323A1

公开(公告)日：2024-01-25

申请号：US17814548

申请日：2022-07-25

Applicant: William M. Gosney ,  Dale B. Nixon

Inventor： William M. Gosney ,  Dale B. Nixon

IPC: G01N15/14

CPC classification number: G01N15/1475 ,  G01N2015/0065

Abstract: An apparatus for locating and venting pathogen cells in blood. A cassette has a plurality of thin holding chambers that are filled with blood drawn from a patient. A light source illuminates each of the holding chambers and passes light to an underlying sensor array such that the cells in the blood produce shadow images of the cells in the sensor array. A processor performs pattern recognition to identify and locate the pathogen cells by use of an image library. After the pathogen cells are located, a pump is operated to move the identified cells to a processing zone. When each identified cell reaches the processing zone, a control voltage is generated to open a valve to vent the identified pathogen cells. The pump refills the cassette holding chambers, returns the processed blood to the patient, and the procedure is repeated for a treatment time period.

公开(公告)号：US11866791B2

公开(公告)日：2024-01-09

申请号：US17940582

申请日：2022-09-08

Applicant: CellMax Ltd.

Inventor： Huangpin B. Hsieh ,  XiaoMing Wang ,  Jr-Ming Lai ,  Rui Mei ,  Hung-Jen Shao ,  Jen-Chia Wu

IPC: G06K9/00 ,  C12Q1/6886 ,  G01N15/14 ,  G01N33/574 ,  G01N33/50 ,  G01N1/31 ,  G01N33/58 ,  C12Q1/6883 ,  G06T7/00

CPC classification number: C12Q1/6886 ,  G01N1/31 ,  G01N15/1475 ,  G01N33/5094 ,  G01N33/57484 ,  G01N33/582 ,  C12Q1/6883 ,  G01N15/1468 ,  G01N2015/1472 ,  G01N2333/4742 ,  G01N2333/70589 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30096 ,  G06T2207/30101

Abstract: Techniques for identifying and enumerating candidate target cells within a biological fluid specimen are described. A digital image of the biological fluid specimen is received, and one or more candidate regions of pixels in the digital image are identified by identifying connected regions of pixels of a minimum intensity having a size between a minimum size and a maximum size and an aspect ratio that meets a threshold. For each candidate region of at least one of the one or more candidate region, whether the portion of the image corresponding to the candidate region includes more than a threshold number of intensity levels is determined. If the portion of the image corresponding to the candidate region includes more than the threshold number of intensity levels the portion of the image is continued to be treated as a candidate for classification.

公开(公告)号：US11860154B2

公开(公告)日：2024-01-02

申请号：US17331893

申请日：2021-05-27

Applicant: Leuko Labs, Inc.

Inventor： Carlos Castro Gonzalez ,  Ian Butterworth ,  Aurelien Bourquard ,  Alvaro Sanchez Ferro

IPC: G01N33/49 ,  G06T7/00 ,  G06T7/11 ,  G06T7/38 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N21/31 ,  G06V20/69 ,  G06F18/214 ,  G06V10/764 ,  G06V10/82 ,  G06V10/56 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N33/49 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1475 ,  G01N21/31 ,  G06F18/2148 ,  G06T7/0016 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/695 ,  G06V20/698 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N2015/008 ,  G01N2015/0073 ,  G01N2015/1006 ,  G01N2015/1443 ,  G01N2015/1486 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30101 ,  G06T2207/30242 ,  G06V2201/03

Abstract: In one aspect, a method to detect white blood cells and/or white blood cell subtypes from non-invasive capillary videos is featured. The method includes acquiring a first plurality of images of a region of interest including one or more capillaries of a predetermined area of a human subject from non-invasive capillary videos captured with an optical device, processing the first plurality of images to determine one or more optical absorption gaps located in said capillary, and annotating the first plurality of images with an indication of any optical absorption gap detected in the first plurality of images. The method also includes acquiring a second plurality of images of the same region of interest of the same capillary with an advanced optical device capable of resolving cellular structure of white blood cells and white blood cell subtypes and spatiotemporally annotating the second plurality of images with an indication of any white blood cell detected and/or a subtype of any white blood cell detected in the second plurality of images. The method also includes inputting the first plurality of images and annotated information from the first plurality of images and annotated information from the spatiotemporally annotated second plurality of images into a machine learning subsystem configured to determine a presence of white blood cells and/or the subtype of any white blood cells present in the one or more optical absorption gaps in the first plurality of images.

公开(公告)号：US11781987B2

公开(公告)日：2023-10-10

申请号：US16831124

申请日：2020-03-26

Applicant: SYSMEX CORPORATION

Inventor： Momoko Imakubo

IPC: G06K9/00 ,  G01N21/64 ,  G01N15/14 ,  G06V20/20 ,  G06V20/69

CPC classification number: G01N21/6456 ,  G01N15/1475 ,  G06V20/20 ,  G06V20/693 ,  G01N2015/1486

Abstract: Disclosed is a fluorescence image analyzer for analyzing a fluorescence image of a cell contained in a sample. The fluorescence image analyzer includes a light source configured to apply light to the sample; an imaging unit configured to capture a fluorescence image of the cell by which fluorescence is generated by applying the light; and a processing unit configured to process the captured fluorescence image. The processing unit is programmed to obtain a bright point pattern of fluorescence in the captured fluorescence image; select at least one bright point pattern, from among a plurality of bright point patterns that include one or more positive patterns and are associated with at least one of a measurement item or a labeling reagent; and determine what positive pattern, among the selected at least one bright point pattern, corresponds to the obtained bright point pattern, based on the obtained bright point pattern and the selected at least one bright point pattern.