公开(公告)号：US06596486B2

公开(公告)日：2003-07-22

申请号：US09758735

申请日：2001-01-11

申请人： Maxim D. Frank-Kamenetskii ,  Nikolay O. Bukanov ,  Vadim V. Demidov ,  Heiko Kuhn ,  Natalia E. Broude

发明人： Maxim D. Frank-Kamenetskii ,  Nikolay O. Bukanov ,  Vadim V. Demidov ,  Heiko Kuhn ,  Natalia E. Broude

IPC分类号： C12Q168

CPC分类号： C12Q1/6839 ,  C12Q1/6832 ,  C12Q2525/307 ,  C12Q2525/107 ,  C12Q2535/101 ,  C12Q2535/119

摘要： A stable complex, we refer to as a PD-Loop, between double stranded nucleic acid and a nucleobase polymer is assembled with the aid of strand invading peptide nucleic acid (PNA). The PD-Loop can be used in the detection, analysis, quantitation and even in the affinity capture of the duplex nucleic acid. Alternatively, the PD-Loop can be used to initiate polymerase extension of a primer to thereby facilitate sequencing of the double stranded nucleic acid even in the presence of large excesses of unrelated double stranded nucleic acid. As an additional feature, the PD-Loop can also be used to generate a construct comprised of a double stranded nucleic acid through which is threaded a single stranded dosed circular nucleic acid wherein the closed circular nucleic acid can be used in a signal amplification methodology.

摘要翻译： 借助于链入侵肽核酸（PNA），将双链核酸和核碱基聚合物之间的一个稳定的复合物称为PD-环。 PD-Loop可用于双相核酸的检测，分析，定量甚至亲和力捕获。 或者，PD-Loop可用于启动引物的聚合酶延伸，从而即使在大量过量的不相关双链核酸的存在下也促进双链核酸的测序。 作为另外的特征，PD-Loop还可以用于产生由双链核酸组成的构建体，通过该双链核酸连接单链计数的环状核酸，其中封闭的环状核酸可以用于信号放大方法。

公开(公告)号：US06265166B1

公开(公告)日：2001-07-24

申请号：US09302201

申请日：1999-04-29

申请人： Maxim D. Frank-Kamenetskii ,  Nikolay O. Bukanov ,  Vadim V. Demidov ,  Heiko Kuhn

发明人： Maxim D. Frank-Kamenetskii ,  Nikolay O. Bukanov ,  Vadim V. Demidov ,  Heiko Kuhn

IPC分类号： C12Q168

CPC分类号： C12Q1/6839 ,  C12Q1/6832 ,  C12Q2525/307 ,  C12Q2525/107 ,  C12Q2535/101 ,  C12Q2535/119

摘要： A stable complex, we refer to as a PD-Loop, between double stranded nucleic acid and a nucleobase polymer is assembled with the aid of strand invading peptide nucleic acid (PNA). The PD-Loop can be used in the detection, analysis, quantitation and even in the affinity capture of the duplex nucleic acid. Alternatively, the PD-Loop can be used to initiate polymerase extension of a primer to thereby facilitate sequencing of the double stranded nucleic acid even in the presence of large excesses of unrelated double stranded nucleic acid. As an additional feature, the PD-Loop can also be used to generate a construct comprised of a double stranded nucleic acid through which is threaded a single stranded closed circular nucleic acid wherein the closed circular nucleic acid can be used in a signal amplification methodology.

摘要翻译： 借助于链入侵肽核酸（PNA），将双链核酸和核碱基聚合物之间的一个稳定的复合物称为PD-环。 PD-Loop可用于双相核酸的检测，分析，定量甚至亲和力捕获。 或者，PD-Loop可用于启动引物的聚合酶延伸，从而即使在大量过量的不相关双链核酸的存在下也促进双链核酸的测序。 作为另外的特征，PD-Loop还可以用于产生由双链核酸组成的构建物，通过该双链核酸连接单链闭环的核酸，其中封闭的环状核酸可以用于信号放大方法。

公开(公告)号：US20120276530A1

公开(公告)日：2012-11-01

申请号：US13391695

申请日：2010-08-24

申请人： Amit Meller ,  Maxim Frank-Kamenetskii ,  Meni Wanunu ,  Heiko Kuhn ,  Alon Singer ,  Will Morrison

发明人： Amit Meller ,  Maxim Frank-Kamenetskii ,  Meni Wanunu ,  Heiko Kuhn ,  Alon Singer ,  Will Morrison

IPC分类号： G01N27/26 ,  B82Y5/00

CPC分类号： C12Q1/6839 ,  C12Q1/689 ,  G01N33/48721 ,  C12Q2525/107

摘要： Embodiments disclosed herein relate to a method of detecting specific DNA sequences and the application of this method in the detection of pathogens, viruses, drug-resistant pathogens, genomic variations associated with disease/disorder susceptibility etc. based on specific signature sequences unique to the pathogens, viruses, drug-resistant pathogens or genomic variations. The method can also be used to distinguish a pool of same-sized dsDNA on the basis of sequence differences. The method uses non-optically labeled bis-PNA and/or gamma-PNA probes to tag specific target sequences for identification by solid-state nanopores.

摘要翻译： 本文公开的实施方案涉及检测特定DNA序列的方法，以及该方法在检测病原体，病毒，耐药性病原体，与疾病/病症易感性等相关的基因组变异的基础上的应用，其基于病原体特有的特异性特征序列 ，病毒，耐药病原体或基因组变体。 该方法也可用于根据序列差异区分相同大小的dsDNA库。 该方法使用非光学标记的双-PNA和/或γ-PNA探针来标记特异性靶序列以便通过固态纳米孔鉴定。