公开(公告)号：US06203989B1

公开(公告)日：2001-03-20

申请号：US09276774

申请日：1999-03-25

Applicant: Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

Inventor： Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

IPC: C12Q168

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  Y10S436/807 ,  C12Q2565/501 ,  C12Q2563/131 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2563/143 ,  C12Q2537/125

Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.

公开(公告)号：US06806047B2

公开(公告)日：2004-10-19

申请号：US09776770

申请日：2001-02-06

Applicant: Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

Inventor： Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

IPC: C12Q168

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  Y10S436/807 ,  C12Q2565/501 ,  C12Q2563/131 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2563/143 ,  C12Q2537/125

Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.

公开(公告)号：US06582938B1

公开(公告)日：2003-06-24

申请号：US09854317

申请日：2001-05-11

Applicant: Xing Su ,  Helin Dong ,  Thomas B. Ryder

Inventor： Xing Su ,  Helin Dong ,  Thomas B. Ryder

IPC: C12P1934

CPC classification number: C12Q1/6865

Abstract: The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.

Abstract translation: 本发明涉及核酸，优选mRNA的扩增。 将引物和启动子加入要扩增的靶序列中，然后在体外转录反应中扩增靶。