公开(公告)号：US5942415A

公开(公告)日：1999-08-24

申请号：US713636

申请日：1996-09-13

申请人： Burkhart Schraven ,  Anne Marie-Cardine ,  Stefan Meuer ,  Henning Kirchgessner

发明人： Burkhart Schraven ,  Anne Marie-Cardine ,  Stefan Meuer ,  Henning Kirchgessner

IPC分类号： C07K14/47 ,  C12P21/06 ,  C07H21/04 ,  C12N1/20

CPC分类号： C07K14/47 ,  A01K2217/05

摘要： Isolated nucleic acid molecules encoding a novel protein, SKAP55, that interacts with the protein tyrosine kinase Fyn, are disclosed. SKAP55 protein has an apparent native molecular weight of 55 kDa, an isoelectric point of 4.3 and contains a pleckstrin homology (PH) domain and a src homology 3 (SH3) domain. In addition to isolated nucleic acids molecules encoding SKAP55 protein, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a SKAP55 transgene. The invention further provides isolated SKAP55 proteins and peptides, SKAP55 fusion proteins and anti-SKAP55 antibodies. Methods of using the SKAP55 compositions of the invention are also disclosed, including methods for detecting SKAP55 protein or mRNA in a biological sample, methods of modulating SKAP55 activity in a cell, and methods for identifying agents that modulate an interaction between SKAP55 and Fyn.

摘要翻译： 公开了编码与蛋白酪氨酸激酶Fyn相互作用的新蛋白质SKAP55的分离的核酸分子。 SKAP55蛋白具有55kDa的表观天然分子量，等电点为4.3，并含有pleckstrin同源性（PH）结构域和src同源性3（SH3）结构域。 除了编码SKAP55蛋白的分离的核酸分子之外，本发明还提供了反义核酸分子，含有本发明的核酸分子的重组表达载体，已经引入了表达载体的宿主细胞和携带SKAP55的非人转基因动物 转基因 本发明还提供分离的SKAP55蛋白和肽，SKAP55融合蛋白和抗SKAP55抗体。 还公开了使用本发明的SKAP55组合物的方法，包括用于检测生物样品中的SKAP55蛋白或mRNA的方法，调节细胞中SKAP55活性的方法以及用于鉴定调节SKAP55和Fyn之间相互作用的试剂的方法。