公开(公告)号：US5830737A

公开(公告)日：1998-11-03

申请号：US780869

申请日：1997-01-24

申请人： Margo Anne-Rose Kusters-Van Someren ,  Yvonne Muller ,  Hermanus Cornelis Maria Kester ,  Jacob Visser ,  Albert Johannes Joseph Van Ooyen ,  Claus Rolin

发明人： Margo Anne-Rose Kusters-Van Someren ,  Yvonne Muller ,  Hermanus Cornelis Maria Kester ,  Jacob Visser ,  Albert Johannes Joseph Van Ooyen ,  Claus Rolin

IPC分类号： C12N15/09 ,  C12N9/24 ,  C12N15/11 ,  C12N15/56 ,  C12R1/19 ,  C12R1/66 ,  C12R1/685 ,  C12N9/26

CPC分类号： C12Y302/01015 ,  C12N9/2402

摘要： The exo-polygalacturonase gene from Aspergillus is identified, and the enzyme encoded thereby is expressed. Specifically, the exo-polygalacturonase gene from Aspergillus tubingensis is is cloned and expressed, and DNA sequences from other Aspergillus strains that specifically hybridize therewith are identified. Novel expression vectors that comprise an exo-polygalacturonase encoding sequence, and host cells transformed therewith, are also provided. The invention further relates to the production of recombinant exo-polygalacturonase, and to the use of this protein.

摘要翻译： 鉴定出来自曲霉的外多聚半乳糖醛酸酶基因，并且由此编码的酶被表达。 具体来说，克隆并表达来自曲霉管曲霉的外切多聚半乳糖醛酸酶基因，鉴定与其特异性杂交的其他曲霉菌株的DNA序列。 还提供了包含外切多聚半乳糖醛酸酶编码序列的新型表达载体和用其转化的宿主细胞。 本发明还涉及重组外切聚半乳糖醛酸酶的生产，以及该蛋白质的应用。