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111 条记录 (耗时 0.035 秒)

    Method for preparing anthocyanin oligomers by using coenzyme derived from Aspergillus sp. strain
    4.
    发明授权
    Method for preparing anthocyanin oligomers by using coenzyme derived from Aspergillus sp. strain 有权

    公开(公告)号：US10774351B2

    公开(公告)日：2020-09-15

    申请号：US16046012

    申请日：2018-07-26

    申请人： Pyo-Jam Park ,  Tuk-Rai Jeong ,  Hyun-Pil Yang ,  Jin Woo Hwang

    发明人： Pyo-Jam Park ,  Tuk-Rai Jeong ,  Hyun-Pil Yang ,  Jin Woo Hwang

    IPC分类号： C12P17/16 ,  C12P17/06 ,  C12P19/60 ,  C12N9/24 ,  C12N1/16 ,  C12R1/685

    摘要： The present invention relates to a method of preparing an anthocyanin oligomer using a coenzyme derived from an Aspergillus sp. strain, and more particularly to a method of preparing an anthocyanin oligomer by fermenting an anthocyanin monomer with a coenzyme of Aspergillus niger, which is a kind of Aspergillus sp. strain. According to the present invention, in order to overcome contamination problems during the culturing process using Aspergillus niger, a coenzyme of Aspergillus niger is extracted and the fermentation process is performed using the same, whereby an anthocyanin oligomer characterized by reduced concern of contamination and superior radical-scavenging effects, compared to existing anthocyanin monomers, can be produced. Also, an anthocyanin oligomer, obtained through fermentation using glucosidase as an enzyme contained in the coenzyme, can exhibit excellent fermentation efficiency and radical-scavenging ability, and polymerization of the anthocyanin oligomer can be confirmed even upon the fermentation of the enzyme including glucosidase.

    Enzymatic production of gluconic acid or its salts
    7.
    发明授权
    Enzymatic production of gluconic acid or its salts 失效
    葡萄糖酸或其盐的酶促生产

    公开(公告)号：US5897995A

    公开(公告)日：1999-04-27

    申请号：US765663

    申请日：1997-04-16

    申请人： Albert J. Vroemen ,  Marc Beverini

    发明人： Albert J. Vroemen ,  Marc Beverini

    IPC分类号： C07C59/105 ,  C12N9/04 ,  C12N9/08 ,  C12N9/96 ,  C12P7/58 ,  C12R1/685 ,  C12R1/80 ,  C12P7/64

    CPC分类号： C12N9/0065 ,  C12N9/0006 ,  C12P7/58

    摘要： An enzymatic process for the conversion of glucose into gluconic acid, uses concentrated glucose solutions. The process employs a combination of glucose oxidase and catalase enzymes which may be obtained from an Aspergilltus niger strain and has a high ratio of catalase;glucose oxidase activity. The enzymatic process requires less time than conventional fermentation processes, the yield of the conversion is close to 100% and the obtained gluconic acid/gluconate solutions do not contain impurities.

    摘要翻译： PCT No.PCT / EP96 / 02128 Sec。 371日期1997年04月16日 102（e）日期1997年4月16日PCT提交1996年5月13日PCT公布。 第WO95 / 33631号公报 日期1995年12月14日葡萄糖转化为葡萄糖酸的酶法，使用浓缩葡萄糖溶液。 该方法采用葡萄糖氧化酶和过氧化氢酶的组合，其可以从黑曲霉菌株获得，并且具有高比例的过氧化氢酶;葡萄糖氧化酶活性。 酶法不仅仅是常规发酵方法所需的时间少，转化率接近100％，所得到的葡萄糖酸/葡萄糖酸盐溶液不含杂质。

    Enzyme with .beta.-(1-6)-endoglucanase activity
    9.
    发明授权
    Enzyme with .beta.-(1-6)-endoglucanase activity 失效
    具有β-（1-6） - 葡聚糖酶活性的酶

    公开(公告)号：US5770406A

    公开(公告)日：1998-06-23

    申请号：US745977

    申请日：1996-11-08

    申请人： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Henrik Dalb.o slashed.ge ,  Hans Sejr Olsen

    发明人： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Henrik Dalb.o slashed.ge ,  Hans Sejr Olsen

    IPC分类号： C12G1/00 ,  A23L2/52 ,  C12N1/14 ,  C12N9/24 ,  C12N15/00 ,  C12N15/09 ,  C12P21/00 ,  C12R1/685 ,  C12R1/69 ,  C12R1/885 ,  C12P19/44 ,  C12N1/00 ,  D21C1/00

    CPC分类号： C12Y302/01075 ,  C12N9/2402 ,  Y10S435/945

    摘要： The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译： 本发明涉及由DNA序列编码的β-（1-6） - 内葡聚糖酶活性的酶，该DNA序列a）包含SEQ ID No.3所示的DNA序列，或b）包含DNA的类似物 序列号3所示的序列，其i）与SEQ ID No.3所示的DNA序列同源，和/或ii）与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交， 和/或iii）编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽，和/或iv）编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-（1-6） - 葡聚糖酶，如SEQ ID No.4所示，源自哈茨木霉，CBS 243.71。 本发明还涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。

    Aspergillus niger vacuolar aspartyl protease
    10.
    发明授权
    Aspergillus niger vacuolar aspartyl protease 失效
    黑曲霉液泡天冬氨酰蛋白酶

    公开(公告)号：US5756338A

    公开(公告)日：1998-05-26

    申请号：US731045

    申请日：1996-10-08

    申请人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

    发明人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/62 ,  C12N15/00 ,  C12N15/57 ,  C12N15/58 ,  C12R1/685 ,  C12N15/63 ,  C12N15/80

    CPC分类号： C12N9/62

    摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus aspartic protease, an Aspergillus aspartic protease per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a protease of the aspartic proteinase-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

    摘要翻译： 本发明涉及编码曲霉天冬氨酸蛋白酶，曲霉天门冬氨酸蛋白酶本身的新型DNA序列及其制备方法。 本发明还涉及可用于异源蛋白质表达的天冬氨酸蛋白酶型蛋白酶中缺陷的新型曲霉菌突变株，以及这种突变菌株的制备方法。