公开(公告)号：US20080213906A1

公开(公告)日：2008-09-04

申请号：US12019445

申请日：2008-01-24

申请人： Craig Aurand ,  Charles Mi ,  Paul Ross ,  An Trinh ,  Hillel Brandes ,  Michael Ye

发明人： Craig Aurand ,  Charles Mi ,  Paul Ross ,  An Trinh ,  Hillel Brandes ,  Michael Ye

IPC分类号： G01N33/84

CPC分类号： G01N30/6091 ,  B01J20/06 ,  B01J20/103 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3236

摘要： A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis.

摘要翻译： 描述了使用组合和同时蛋白沉淀和固相萃取（SPE）方法制备用于后续LC-MS分析的生物样品的组合物，方法和装置。 通过使用新型氧化锆涂层色谱介质，蛋白质沉淀，过滤和SPE的综合组合，从生物样品中除去干扰化合物，如蛋白质和含磷酸盐化合物，在LC- MS分析。

公开(公告)号：US10928366B2

公开(公告)日：2021-02-23

申请号：US12019445

申请日：2008-01-24

申请人： Craig Aurand ,  Charles Mi ,  Paul Ross ,  An Trinh ,  Hillel Brandes ,  Michael Ye

发明人： Craig Aurand ,  Charles Mi ,  Paul Ross ,  An Trinh ,  Hillel Brandes ,  Michael Ye

IPC分类号： G01N33/84 ,  G01N30/60 ,  B01J20/10 ,  B01J20/06 ,  B01J20/32 ,  G01N33/00

摘要： A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis.