公开(公告)号：US20120172577A1

公开(公告)日：2012-07-05

申请号：US11909873

申请日：2006-03-31

申请人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Kisaki ,  Yoko Kisaki ,  Hiroyuki Keshi ,  Aya Keshi ,  Hiroshi Ueyama ,  Mizuki Nishihara

发明人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Kisaki ,  Yoko Kisaki ,  Hiroyuki Keshi ,  Aya Keshi ,  Hiroshi Ueyama ,  Mizuki Nishihara

IPC分类号： C07K14/78 ,  C12N15/85 ,  C12N5/10 ,  C12P21/00

CPC分类号： C12P21/02 ,  C07K14/78

摘要： An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods.The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.

摘要翻译： 本发明的目的是提供生产易于分离和纯化的人胶原分子的方法，其具有与天然胶原分子基本上相同的结构，其中用胶原基因转导的宿主细胞合成大量 的衍生自引入高外源基因表达载体的基因的人胶原蛋白。 本发明的另一个目的是提供通过生产方法制备的胶原分子。 本发明人发现，通过从各种哺乳动物细胞中选择具有低胶原表达的宿主细胞并将胶原基因构建体导入能够高度外源的载体中，可以产生几乎不被宿主细胞衍生的胶原蛋白污染的大量人胶原 基因表达。