公开(公告)号：US20120172577A1

公开(公告)日：2012-07-05

申请号：US11909873

申请日：2006-03-31

申请人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Kisaki ,  Yoko Kisaki ,  Hiroyuki Keshi ,  Aya Keshi ,  Hiroshi Ueyama ,  Mizuki Nishihara

发明人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Kisaki ,  Yoko Kisaki ,  Hiroyuki Keshi ,  Aya Keshi ,  Hiroshi Ueyama ,  Mizuki Nishihara

IPC分类号： C07K14/78 ,  C12N15/85 ,  C12N5/10 ,  C12P21/00

CPC分类号： C12P21/02 ,  C07K14/78

摘要： An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods.The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.

摘要翻译： 本发明的目的是提供生产易于分离和纯化的人胶原分子的方法，其具有与天然胶原分子基本上相同的结构，其中用胶原基因转导的宿主细胞合成大量 的衍生自引入高外源基因表达载体的基因的人胶原蛋白。 本发明的另一个目的是提供通过生产方法制备的胶原分子。 本发明人发现，通过从各种哺乳动物细胞中选择具有低胶原表达的宿主细胞并将胶原基因构建体导入能够高度外源的载体中，可以产生几乎不被宿主细胞衍生的胶原蛋白污染的大量人胶原 基因表达。

公开(公告)号：US09018354B2

公开(公告)日：2015-04-28

申请号：US11909873

申请日：2006-03-31

申请人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Ueyama ,  Mizuki Nishihara ,  Yoko Kisaki ,  Aya Keshi

发明人： Tetsuo Kase ,  Akio Kimura ,  Hiroshi Kisaki ,  Hiroyuki Keshi ,  Hiroshi Ueyama ,  Mizuki Nishihara

IPC分类号： A61K38/39 ,  C12P21/02 ,  C07K14/78

CPC分类号： C12P21/02 ,  C07K14/78

摘要： An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods.The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.

摘要翻译： 本发明的目的是提供生产容易分离和纯化的人胶原分子的方法，其具有与天然胶原分子基本相同的结构，其中用胶原基因转导的宿主细胞合成大量 的衍生自引入高外源基因表达载体的基因的人胶原蛋白。 本发明的另一个目的是提供通过生产方法制备的胶原分子。 本发明人发现，通过从各种哺乳动物细胞中选择具有低胶原表达的宿主细胞并将胶原基因构建体导入能够高度外源的载体中，可以产生几乎不被宿主细胞衍生的胶原蛋白污染的大量人胶原 基因表达。

公开(公告)号：US06225453B1

公开(公告)日：2001-05-01

申请号：US09402002

申请日：2000-01-10

申请人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

发明人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

IPC分类号： C07H2104

CPC分类号： C12Q1/689

摘要： The DNA from the bacteria Klebsiella pneumoniae is extracted, then completely digested with restriction enzyme Hind III, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Klebsiella pneumoniae, then the sequence of the probe is elucidated.

摘要翻译： 提取来自肺炎克雷伯杆菌的DNA，然后用限制性内切酶HindIII完全消化，然后克隆到合适的载体中以选择包含基本上包含在肺炎克雷伯杆菌中的DNA的探针，然后阐明探针的序列。

公开(公告)号：US06265568B1

公开(公告)日：2001-07-24

申请号：US09381849

申请日：2000-01-11

申请人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

发明人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

IPC分类号： C07H2104

CPC分类号： C12Q1/689

摘要： The DNA from the bacteria Bactereoides fragilis is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Bacteroides fragilis, then the sequence of the probe is elucidated.

摘要翻译： 提取来自细菌细菌的细菌的DNA，然后用限制酶HindIII完全消化，然后克隆到合适的载体中以选择包含脆弱拟杆菌中基本上含有的DNA的探针，然后阐明探针的序列。

公开(公告)号：US20070059687A1

公开(公告)日：2007-03-15

申请号：US10479027

申请日：2002-05-27

申请人： Tsuneya Ohno ,  Akio Matsuhisa ,  Hiroyuki Keshi ,  Kanako Abe ,  Norihiko Sugimoto ,  Hiroshi Ueyama ,  Soji Eda ,  Hirotsugu Uehara ,  Takahisa Iwami ,  Seiji Yamamoto ,  Hiromasa Araki

发明人： Tsuneya Ohno ,  Akio Matsuhisa ,  Hiroyuki Keshi ,  Kanako Abe ,  Norihiko Sugimoto ,  Hiroshi Ueyama ,  Soji Eda ,  Hirotsugu Uehara ,  Takahisa Iwami ,  Seiji Yamamoto ,  Hiromasa Araki

IPC分类号： C12Q1/68 ,  C12N5/08

CPC分类号： G01N33/5047 ,  C12Q1/6841 ,  C12Q1/689 ,  G01N33/5005

摘要： A digested phagocyte prepared by contacting in vitro a phagocyte with a foreign microorganism and isolating the phagocyte so contacted; a process for producing the same; and a process and a kit in which these are utilized are disclosed. An experimental model, which enables in vitro evaluation of a phagocytotic function of phagocytes, is provided.

摘要翻译： 通过体外将吞噬细胞与外来微生物接触并分离所接触的吞噬细胞制备的消化的吞噬细胞; 其制造方法; 并且公开了使用这些的方法和试剂盒。 提供了能够体外评估吞噬细胞吞噬功能的实验模型。

公开(公告)号：US06660852B1

公开(公告)日：2003-12-09

申请号：US08809254

申请日：1997-05-16

申请人： Hiroyuki Keshi ,  Soji Eda ,  Hirotsugu Uehara ,  Hiroshi Ueyama ,  Akio Matsuhisa

发明人： Hiroyuki Keshi ,  Soji Eda ,  Hirotsugu Uehara ,  Hiroshi Ueyama ,  Akio Matsuhisa

IPC分类号： C12N1563

CPC分类号： C12Q1/689

摘要： A probe derived from bacteria of pneumonia, containing fragments of DNA which streptococcus pneumoniae essentially possesses, and useful for detecting and identifying causative bacteria of pneumonia is obtained by completely digesting the DNA with a restriction endonuclease PstI, followed by cloning into a suitable vector.

摘要翻译： 通过用限制性内切核酸酶PstI完全消化DNA，然后克隆到合适的载体中，获得含有肺炎链球菌基本上具有的DNA片段的可用于检测和鉴定肺炎的致病菌的肺炎细菌的探针。

公开(公告)号：US06245906B1

公开(公告)日：2001-06-12

申请号：US09381862

申请日：2000-01-11

申请人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

发明人： Hiroshi Ueyama ,  Kanako Abe ,  Hiroyuki Keshi ,  Akio Matsuhisa

IPC分类号： C07H2104

CPC分类号： C12Q1/689 ,  C07K14/315

摘要： The DNA from the bacteria Streptococcus pyogenes is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Streptococcus pyogenes, then the sequence of the probe is elucidated.

摘要翻译： 提取来自细菌化脓性链球菌的DNA，然后用限制性内切酶HindIII完全消化，然后克隆到合适的载体中以选择包含在化脓性链球菌中基本上含有的DNA的探针，然后阐明探针的序列。

公开(公告)号：US20050099574A1

公开(公告)日：2005-05-12

申请号：US10948758

申请日：2004-09-24

申请人： Hiroshi Ueyama

发明人： Hiroshi Ueyama

IPC分类号： G02F1/13 ,  G02F1/1333 ,  G02F1/1339 ,  G02F1/1341

CPC分类号： G02F1/1341 ,  G02F1/133351 ,  G02F1/1339 ,  G02F2001/13415

摘要： The invention aims at improving the uniformity in gap between an array substrate and a color filter substrate of a liquid crystal display device to be manufactured by a multi-panel forming process filling liquid crystal by a liquid crystal dripping method. A liquid crystal display device of the invention comprises an array substrate having a plurality of first seal frames each forming a panel area to become a display portion and a second seal frame containing these seal frames thereinside and being of a completely closed shape as a whole outside these first seal frames. The liquid crystal display device of the invention has a structure in which the whole inside of the second seal frame including the insides of the first seal frames of the array substrate is filled with liquid crystal by a liquid crystal dripping method and thereafter the color filter substrate is stuck on the array substrate. The invention makes it possible to manufacture a liquid crystal display device of an aggregate liquid crystal display panel structure improved in uniformity of gap between both substrates by means of this structure.

摘要翻译： 本发明的目的在于提高通过液晶滴加方法通过多面板成形方法填充液晶来制造的液晶显示装置的阵列基板和滤色器基板之间的间隙的均匀性。 本发明的液晶显示装置包括：阵列基板，具有多个第一密封框架，每个第一密封框架都形成面板区域以成为显示部分;以及第二密封框架，其包含位于其下方的这些密封框架，并且整体处于完全封闭的形状 这些第一个密封框架。 本发明的液晶显示装置具有这样的结构，其中包括阵列基板的第一密封框架的内部的第二密封框架的整个内部通过液晶滴落法填充液晶，然后滤色器基板 卡在阵列基板上。 本发明可以制造通过该结构提高两基板之间的间隙均匀性的集合液晶显示面板结构的液晶显示装置。

公开(公告)号：US07193678B2

公开(公告)日：2007-03-20

申请号：US10948758

申请日：2004-09-24

申请人： Hiroshi Ueyama

发明人： Hiroshi Ueyama

IPC分类号： G02F1/1339

CPC分类号： G02F1/1341 ,  G02F1/133351 ,  G02F1/1339 ,  G02F2001/13415

摘要： A liquid crystal display device includes an array substrate having a plurality of first seal frames each forming a panel area to become a display portion and a second seal frame containing these seal frames thereinside and being of a completely closed shape as a whole outside these first seal frames. The liquid crystal display device of the invention has a structure in which the whole inside of the second seal frame including the insides of the first seal frames of the array substrate is filled with liquid crystal by a liquid crystal dripping method and thereafter the color filter substrate is stuck on the array substrate. This display makes it possible to manufacture a liquid crystal display device of an aggregate liquid crystal display panel structure improved in uniformity of gap between both substrates.

摘要翻译： 液晶显示装置包括阵列基板，该阵列基板具有多个第一密封框架，每个第一密封框架形成面板以成为显示部分;以及第二密封框架，其内部包含这些密封框架，并且在这些第一密封件外面整体上处于完全闭合的形状 框架。 本发明的液晶显示装置具有这样的结构，其中包括阵列基板的第一密封框架的内部的第二密封框架的整个内部通过液晶滴落法填充液晶，然后滤色器基板 卡在阵列基板上。 通过该显示，可以制造提高两基板之间的间隙均匀性的集合液晶显示面板结构的液晶显示装置。