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公开(公告)号：US08911721B2
公开(公告)日：2014-12-16
申请号：US12301670
申请日：2007-05-24
申请人： Yuko Shimatani , Yoshinori Hamaji , Hitomi Matsuhashi , Jun Amano , Shun'ichiro Taniguchi , Minoru Fujimori
发明人： Yuko Shimatani , Yoshinori Hamaji , Hitomi Matsuhashi , Jun Amano , Shun'ichiro Taniguchi , Minoru Fujimori
CPC分类号： A61K48/0008 , A61K31/513 , A61K35/74 , A61K35/745 , A61K48/00 , C12N9/78 , Y02A50/473
摘要： An object of the present invention is to provide a method of efficiently constructing a gene delivery carrier having a favorable activity and expression efficiency of a protein expressed by a gene introduced by transformation. Moreover, an object of the present invention is to provide a pharmaceutical composition comprising a gene delivery carrier constructed by the construction method and a therapeutic agent for solid tumor comprising the resistant bacterium. The objects of the present invention have been attained by a gene delivery carrier consisting of an anaerobic microorganism capable of growing in a tumor tissue in an anaerobic environment and capable of expressing (A) a protein having an antitumor activity or (B) a protein having an activity of converting an antitumor substance precursor to an antitumor substance, wherein DNA encoding the protein of interest is allowed to further comprise, on the 5′-terminal side thereof, a DNA fragment comprising a nucleotide sequence encoding a peptide consisting of an amino acid sequence from the 1st to at least 4th amino acids at the N-terminus of a histone-like DNA-binding protein.
摘要翻译： 本发明的目的是提供一种有效地构建由通过转化引入的基因表达的蛋白质具有良好活性和表达效率的基因递送载体的方法。 此外，本发明的目的是提供一种药物组合物，其包含通过构建方法构建的基因递送载体和包含耐药性细菌的固体肿瘤治疗剂。 本发明的目的是通过一种基因递送载体实现的，所述基因递送载体由厌氧微生物组成，所述厌氧微生物能够在厌氧环境中在肿瘤组织中生长并且能够表达（A）具有抗肿瘤活性的蛋白质，或（B）具有 将抗肿瘤物质前体转化为抗肿瘤物质的活性，其中编码目的蛋白质的DNA在其5'-末端侧进一步包含编码由氨基酸组成的肽的核苷酸序列的DNA片段 序列从组蛋白样DNA结合蛋白的N末端的第1个至第4个氨基酸。
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公开(公告)号：US20090169516A1
公开(公告)日：2009-07-02
申请号：US12301670
申请日：2007-05-24
申请人： Yuko Shimatani , Yoshinori Hamaji , Hitomi Matsuhashi , Jun Amano , Shun'ichiro Taniguchi , Minoru Fujimori
发明人： Yuko Shimatani , Yoshinori Hamaji , Hitomi Matsuhashi , Jun Amano , Shun'ichiro Taniguchi , Minoru Fujimori
CPC分类号： A61K48/0008 , A61K31/513 , A61K35/74 , A61K35/745 , A61K48/00 , C12N9/78 , Y02A50/473
摘要： An object of the present invention is to provide a method of efficiently constructing a gene delivery carrier having a favorable activity and expression efficiency of a protein expressed by a gene introduced by transformation. Moreover, an object of the present invention is to provide a pharmaceutical composition comprising a gene delivery carrier constructed by the construction method and a therapeutic agent for solid tumor comprising the resistant bacterium. The objects of the present invention have been attained by a gene delivery carrier consisting of an anaerobic microorganism capable of growing in a tumor tissue in an anaerobic environment and capable of expressing (A) a protein having an antitumor activity or (B) a protein having an activity of converting an antitumor substance precursor to an antitumor substance, wherein DNA encoding the protein of interest is allowed to further comprise, on the 5′-terminal side thereof, a DNA fragment comprising a nucleotide sequence encoding a peptide consisting of an amino acid sequence from the 1st to at least 4th amino acids at the N-terminus of a histone-like DNA-binding protein.
摘要翻译： 本发明的目的是提供一种有效地构建由通过转化引入的基因表达的蛋白质具有良好活性和表达效率的基因递送载体的方法。 此外，本发明的目的是提供一种药物组合物，其包含通过构建方法构建的基因递送载体和包含耐药性细菌的固体肿瘤治疗剂。 本发明的目的是通过一种基因递送载体实现的，所述基因递送载体由厌氧微生物组成，所述厌氧微生物能够在厌氧环境中在肿瘤组织中生长并且能够表达（A）具有抗肿瘤活性的蛋白质，或（B）具有 将抗肿瘤物质前体转化为抗肿瘤物质的活性，其中编码目的蛋白质的DNA在其5'-末端侧进一步包含编码由氨基酸组成的肽的核苷酸序列的DNA片段 序列从组蛋白样DNA结合蛋白的N末端的第1个至第4个氨基酸。
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