公开(公告)号：US20070059764A1

公开(公告)日：2007-03-15

申请号：US11229431

申请日：2005-09-15

Applicant: Matthew Hart ,  Horn Lin ,  Jay Eversole

Inventor： Matthew Hart ,  Horn Lin ,  Jay Eversole

IPC: G01N33/53 ,  C12M1/34

CPC classification number: G01N33/54386 ,  G01N15/1459 ,  G01N2015/0046

Abstract: A device having an air sampler, an electrospray apparatus, and a fluorescence excitation and detection system. The air sampler is capable of moving air suspected of containing a biological or chemical aerosol particle into a chamber. The electrospray apparatus is capable of spraying a charged solution into the chamber to coat the aerosol particles with a coating. The solution has a fluorescent-labeled biological or chemical marker capable of specific binding to the aerosol particle. The fluorescence system is capable of detecting fluorescence of the fluorescent label in the coating. A method of detecting the aerosol particle by: moving air suspected of containing the aerosol particle into a chamber; spraying the charged solution into the chamber with an electrospray apparatus, such that a coating of the solution is formed around the particle; exciting the fluorescent label; and detecting fluorescence of the fluorescent label.

Abstract translation: 具有空气采样器，电喷射装置和荧光激发和检测系统的装置。 空气取样器能够将怀疑含有生物或化学气溶胶颗粒的空气移动到室中。 电喷射装置能够将带电溶液喷射到室中以用涂层涂覆气溶胶颗粒。 该溶液具有能够特异性结合气溶胶颗粒的荧光标记的生物或化学标记。 荧光系统能够检测涂层中荧光标记的荧光。 一种通过以下步骤检测气溶胶颗粒的方法：将怀疑含有气溶胶颗粒的空气移动到室中; 用电喷雾装置将带电溶液喷射到室中，使得在颗粒周围形成溶液的涂层; 激发荧光标签; 并检测荧光标记物的荧光。