公开(公告)号：US08372961B2

公开(公告)日：2013-02-12

申请号：US12692392

申请日：2010-01-22

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： C12N15/12

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US07670602B2

公开(公告)日：2010-03-02

申请号：US11739288

申请日：2007-04-24

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： A61K39/395

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US20090011466A1

公开(公告)日：2009-01-08

申请号：US11739288

申请日：2007-04-24

申请人： Yong-Hoon CHUNG ,  Ji-Woong HAN ,  Hye-Ja LEE ,  Eun-Yong CHOI ,  Jin-Mi KIM

发明人： Yong-Hoon CHUNG ,  Ji-Woong HAN ,  Hye-Ja LEE ,  Eun-Yong CHOI ,  Jin-Mi KIM

IPC分类号： C12P21/02 ,  C07K14/54 ,  C12N15/62 ,  C12N15/85 ,  C12N5/10

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US20100278827A1

公开(公告)日：2010-11-04

申请号：US12692392

申请日：2010-01-22

申请人： Yong-Hoon CHUNG ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon CHUNG ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： A61K39/395 ,  C07K16/46 ,  C07H21/04 ,  C12N15/63 ,  C12N5/07 ,  C12P21/02 ,  A61P37/00

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment pf an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端连接到生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端。 此外，本发明公开了通过在两个单体蛋白质的铰链区上形成分子间二硫键而形成的二聚体蛋白质，这两个单体蛋白质是通过将免疫应答的两个相同的可溶性细胞外区域与免疫球蛋白分子的Fc片段连接起来而形成的， 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US07229962B2

公开(公告)日：2007-06-12

申请号：US10363427

申请日：2002-07-26

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： C07K14/715 ,  A61K38/16

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。