公开(公告)号：US4418060A

公开(公告)日：1983-11-29

申请号：US76337

申请日：1979-09-17

申请人： Ilona Kahan nee Laszlo ,  Helga Hammer ,  Ilona Beladi

发明人： Ilona Kahan nee Laszlo ,  Helga Hammer ,  Ilona Beladi

IPC分类号： A61K31/65 ,  A61K20060101 ,  A61K31/13 ,  C07C20060101 ,  C07C67/00 ,  C07C231/00 ,  C07C231/22 ,  C07C237/26 ,  C07C103/19

CPC分类号： C07D277/42

摘要： Tri-tetracyclines are complexes of tetracyclines with an amino compound containing per molecule at least a tris (hydroxyalkyl) group and at least a primary or secondary amino group in a molar excess in respect to the tetracycline compound. The complexes have high water solubility and are particularly useful for parenteral administration.

摘要翻译： 四环素是四环素与每分子含有至少三（羟基烷基）基团的氨基化合物和至少相对于四环素化合物摩尔过量的伯或仲氨基的复合物。 该复合物具有高水溶性，并且特别适用于肠胃外给药。

公开(公告)号：US4696899A

公开(公告)日：1987-09-29

申请号：US681451

申请日：1984-12-10

申请人： Miklos Toth ,  Valeria Endresz ,  Ilona Beladi ,  Sandor Toth

发明人： Miklos Toth ,  Valeria Endresz ,  Ilona Beladi ,  Sandor Toth

IPC分类号： C12P21/00 ,  A61K35/14 ,  A61K38/21 ,  A61P43/00 ,  C07K1/14 ,  C07K1/22 ,  C07K14/52 ,  C07K14/555 ,  C07K14/56 ,  C07K14/57 ,  C07K16/00 ,  C12P21/02 ,  C12R1/91 ,  A61K45/02

CPC分类号： C07K14/555 ,  Y10S435/811

摘要： The invention relates to a process for the preparation of .alpha.- and .gamma.-interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an .alpha.-interferon inducer and with a .gamma.-interferon inducer, which comprises producing .alpha.-interferon and .gamma.-interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/buffy coat/fraction of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with - or .beta.-interferon, contacting with an .alpha.-interferon inducer, separating the liquid containing the .alpha.-interferon from the cells, if desired recovering the -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising .alpha.-interferon from the cells and if desired recovering .alpha.-interferon and if desired making .alpha.-interferon free of .alpha.-interferon.The advantage of the process is that .alpha.- and .gamma.-interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available leukocytes.

摘要翻译： 本发明涉及通过分离血液的血沉棕黄层级分，去除红细胞，将白细胞悬浮在合适的营养培养基中并用α-干扰素诱导剂和γ-干扰素诱导剂进行处理的方法来制备α-和γ-干扰素， 干扰素诱导剂，其包括通过预处理在分离白细胞/血沉棕黄层/血液部分之后获得的悬浮液，在相同的白细胞培养物中逐渐生成α-干扰素和γ-干扰素，去除红细胞并悬浮 在具有β-干扰素的合适营养培养基中的白细胞与α-干扰素诱导剂接触，如果需要，将含有α-干扰素的液体与细胞分离，如果需要，则回收 - 干扰素，洗涤并将细胞悬浮在合适的营养培养基 用有丝分裂剂处理，将含有α-干扰素的液体与细胞分离，并且如果需要，可以回收α-inter feron，如果需要，制造不含α-干扰素的α-干扰素。 该方法的优点是α-和γ-干扰素是在两个步骤中由相同的白细胞培养产生的。 这使得该方法更经济并能有效利用有限可用的白细胞。