公开(公告)号：US06555651B2

公开(公告)日：2003-04-29

申请号：US08948131

申请日：1997-10-09

申请人： David Stern ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

发明人： David Stern ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

IPC分类号： A61K3800

CPC分类号： C07K14/70503 ,  A01K2217/075 ,  A61K38/00

摘要： The present method provides for an isolated peptide having an amino acid sequence corresponding to the amino acid sequence of a V-domain of a receptor for advanced glycation endproduct (RAGE). The present invention also provides for an isolated peptide having an amino acid sequence A-Q-N-I-T-A-R-I-G-E-P-L-V-L-K-C-K-G-A-P-K-K-P-P-Q-R-L-E-W-K (SEQ. ID No: 1). The present invention provides for a pharmaceutical composition comprising a therapeutically effect amount of an isolated peptide having an amino acid sequence corresponding to the amino acid sequence of a V-domain of RAGE. The present invention also provides for a method for inhibiting interaction of an amyloid-&bgr; peptide with a receptor for advanced glycation end product which is on the surface of a cell, which comprises contacting the cell with the peptide or a functionally equivalent agent, wherein the peptide or agent is capable of inhibiting interaction of the amyloid-&bgr; peptide with the receptor for advanced glycation end product, and the peptide or agent is present in an amount effective to inhibit interaction of the amyloid-&bgr; peptide with the receptor for advanced glycation endproduct.

摘要翻译： 本方法提供了具有对应于晚期糖基化终产物（RAGE）受体的V-结构域的氨基酸序列的氨基酸序列的分离的肽。 本发明还提供具有氨基酸序列A-Q-N-I-T-A-R-I-G-E-P-L-V-L-K-C-K-G-A-P-K-P-P-Q-R-L-E-W-K（SEQ ID NO：1）的分离肽。 本发明提供了一种药物组合物，其包含治疗有效量的具有对应于RAGE的V-结构域的氨基酸序列的氨基酸序列的分离的肽。 本发明还提供了抑制淀粉样蛋白-β肽与细胞表面上的晚期糖基化终产物的受体的相互作用的方法，其包括使细胞与肽或功能等同试剂接触，其中 肽或试剂能够抑制淀粉样蛋白β肽与晚期糖基化终产物的受体的相互作用，并且肽或试剂以有效抑制淀粉样蛋白-β肽与晚期糖基化终产物受体相互作用的量存在 。

公开(公告)号：US20050170382A1

公开(公告)日：2005-08-04

申请号：US10990310

申请日：2004-11-15

申请人： David Stern ,  Kevan Herold ,  Shi Yan ,  Ann Schmidt ,  Ira Lamster

发明人： David Stern ,  Kevan Herold ,  Shi Yan ,  Ann Schmidt ,  Ira Lamster

IPC分类号： A61K31/00 ,  A61K38/17 ,  C07K16/18 ,  C12Q1/68 ,  A01K67/00 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28

CPC分类号： A61K31/00 ,  A61K38/1774 ,  A61K2039/505 ,  C07K16/18

摘要： The present invention provides for an isolated human EN-RAGE peptide. The present invention also provides for a method for determining whether a compound is capable of inhibiting the interaction of an EN-RAGE peptide with a RAGE peptide, which comprises: (a) admixing: (i) a RAGE peptide or an sRAGE peptide or a fragment of either thereof, (ii) an EN-RAGE peptide or a fragment thereof, and (iii) the compound; (b) measuring the level of interaction between the peptide of step (a) (i) and the peptide of step (a) (ii), and (c) comparing the amount of interaction meausred in step (b) with the amount measured between the petpide of step (a) (i) and the peptide of step (a) (ii) in the absence of the compound, thereby determining whether the compound is capable of inhibiting the interaction of the EN-RAGE peptide with the RAGE peptide, wherein a reduction in the amount of interaction in the presence of the compound indicates that the compound is capable of inhibiting the interaction. The present invention also provides for a method for inhibiting inflammation in a subject which comprises administering to the subject a compound capable of interfering with the interaction between EN-RAGE peptide and receptor for advanced glycation endproduct (RAGE) in the subject thereby inhibiting inflammation in the subject.

摘要翻译： 本发明提供了分离的人类EN-RAGE肽。 本发明还提供了一种用于确定化合物是否能够抑制EN-RAGE肽与RAGE肽的相互作用的方法，其包括：（a）混合：（i）RAGE肽或sRAGE肽或 片段，（ii）EN-RAGE肽或其片段，和（iii）化合物; （b）测量步骤（a）（i）的肽与步骤（a）（ii）的肽之间的相互作用水平，和（c）将步骤（b）中所发生的相互作用的量与测量的量进行比较 在不存在化合物的情况下，步骤（a）（i）的petpide和步骤（a）（ii）的肽之间，从而确定化合物是否能够抑制EN-RAGE肽与RAGE肽的相互作用 其中在化合物存在下相互作用量的减少表明该化合物能够抑制相互作用。 本发明还提供了一种抑制受试者的炎症的方法，其包括向受试者施用能够干扰受试者的EN-RAGE肽和受体之间相互作用的晚期糖基化终产物（RAGE）的相互作用，从而抑制受试者的炎症 学科。

公开(公告)号：US07258857B2

公开(公告)日：2007-08-21

申请号：US09872185

申请日：2001-06-01

申请人： David M. Stern ,  Kevan Herold ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

发明人： David M. Stern ,  Kevan Herold ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

IPC分类号： A61K39/395 ,  C07K16/28

CPC分类号： A61K31/00 ,  A61K38/1774 ,  A61K2039/505 ,  C07K16/18 ,  Y10S514/825

摘要： The present invention provides a method for treating inflammation in a subject which comprises administering to the subject soluble receptor for advanced glycation endproduct (sRAGE) in an amount effective to inhibit binding of advanced glycation endproducts (AGEs) to RAGE thereby treating inflammation in the subject. The present invention also provides for a method for treating inflammation in a subject which comprises administering to the subject an agent in an amount effective to inhibit the interaction between receptor for advanced glycation endproduct (RAGE) and its ligand thereby treating inflammation in the subject.

摘要翻译： 本发明提供了一种用于治疗受试者的炎症的方法，其包括以有效抑制晚期糖基化终产物（AGE）与RAGE结合的量向受试者可溶性受体施用晚期糖基化终产物（sRAGE），从而治疗受试者的炎症。 本发明还提供了一种用于治疗受试者的炎症的方法，其包括以有效抑制晚期糖基化终产物（RAGE）的受体与其配体之间的相互作用的量向受试者施用试剂，由此治疗受试者的炎症。

公开(公告)号：US20080214453A1

公开(公告)日：2008-09-04

申请号：US11894503

申请日：2007-08-20

申请人： David M. Stern ,  Kevan Herold ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

发明人： David M. Stern ,  Kevan Herold ,  Shi Du Yan ,  Ann Marie Schmidt ,  Ira Lamster

IPC分类号： A61K38/16 ,  A61P29/00

CPC分类号： A61K31/00 ,  A61K38/1774 ,  A61K2039/505 ,  C07K16/18 ,  Y10S514/825

摘要： The present invention provides a method for treating inflammation in a subject which comprises administering to the subject soluble receptor for advanced glycation endproduct (sRAGE) in an amount effective to inhibit binding of advanced glycation endproducts (AGEs) to RAGE thereby treating inflammation in the subject. The present invention also provides for a method for treating inflammation in a subject which comprises administering to the subject an agent in an amount effective to inhibit the interaction between receptor for advanced glycation endproduct (RAGE) and its ligand thereby treating inflammation in the subject.

摘要翻译： 本发明提供了一种用于治疗受试者的炎症的方法，其包括以有效抑制晚期糖基化终产物（AGE）与RAGE结合的量向受试者可溶性受体施用晚期糖基化终产物（sRAGE），从而治疗受试者的炎症。 本发明还提供了一种用于治疗受试者的炎症的方法，其包括以有效抑制晚期糖基化终产物（RAGE）的受体与其配体之间的相互作用的量向受试者施用试剂，由此治疗受试者的炎症。