公开(公告)号：US09464314B2

公开(公告)日：2016-10-11

申请号：US13447606

申请日：2012-04-16

申请人： Dominique Poncelet ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

发明人： Dominique Poncelet ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

IPC分类号： G01N1/44 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/301

摘要： A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.

摘要翻译： 将具有至少10000碱基对的起始大小的DNA序列片段化为平均大小小于或等于1300bp的片段的方法，其中将DNA序列置于溶液中，将包含DNA序列的溶液置于 将容器和容器放置在经受超声波作用的液浴中，使得超声波穿过液浴以激发容器和溶液以剪切DNA序列，并且其中超声波 频率落在28 kHz和80 kHz之间的范围内。

公开(公告)号：US20120264228A1

公开(公告)日：2012-10-18

申请号：US13447606

申请日：2012-04-16

申请人： Dominique PONCELET ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

发明人： Dominique PONCELET ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

IPC分类号： G01N1/44

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/301

摘要： A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.

摘要翻译： 将具有至少10000碱基对的起始大小的DNA序列片段化为平均大小小于或等于1300bp的片段的方法，其中将DNA序列置于溶液中，将包含DNA序列的溶液置于 将容器和容器放置在经受超声波作用的液浴中，使得超声波穿过液浴以激发容器和溶液以剪切DNA序列，并且其中超声波 频率落在28 kHz和80 kHz之间的范围内。