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    Fragmenting DNA sequences longer than 10,000 BP using ultrasonication
    4.
    发明授权
    Fragmenting DNA sequences longer than 10,000 BP using ultrasonication 有权
    使用超声波分离长于10,000 BP的DNA序列

    公开(公告)号：US09464314B2

    公开(公告)日：2016-10-11

    申请号：US13447606

    申请日：2012-04-16

    申请人： Dominique Poncelet ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

    发明人： Dominique Poncelet ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

    IPC分类号： G01N1/44 ,  C12Q1/68

    CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/301

    摘要： A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.

    摘要翻译： 将具有至少10000碱基对的起始大小的DNA序列片段化为平均大小小于或等于1300bp的片段的方法，其中将DNA序列置于溶液中，将包含DNA序列的溶液置于 将容器和容器放置在经受超声波作用的液浴中，使得超声波穿过液浴以激发容器和溶液以剪切DNA序列，并且其中超声波 频率落在28 kHz和80 kHz之间的范围内。

    LONG INSERT-BASED WHOLE GENOME SEQUENCING
    6.
    发明申请
    LONG INSERT-BASED WHOLE GENOME SEQUENCING 审中-公开
    长基于插入的全基因测序

    公开(公告)号：US20150126379A1

    公开(公告)日：2015-05-07

    申请号：US14526344

    申请日：2014-10-28

    申请人： THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

    发明人： Winnie Liang ,  John Carpten ,  David Craig

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q2523/301 ,  C12Q2535/122 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/149

    摘要： The present invention is directed to a method of detecting a genomic rearrangement in a nucleic acid sample with Long Insert Whole Genome Sequencing (LI-WGS). The method may include obtaining a nucleic acid sample and then fragmenting the nucleic acid sample (e.g., via sonication). In particular, the fragmenting may result in the production of a plurality of inserts. Thereafter, the method comprises purifying the plurality of inserts using magnetic beads and then amplifying the purified plurality of inserts. In addition, the method further comprises sequencing the purified and amplified plurality of inserts. In some aspects, the plurality of inserts have a length of between about 800 and about 1,100 base pairs.

    摘要翻译： 本发明涉及用长插入全基因组测序（LI-WGS）检测核酸样品中的基因组重排的方法。 该方法可以包括获得核酸样品，然后将核酸样品（例如通过超声处理）分段。 特别地，碎片可能导致生产多个插入物。 此后，该方法包括使用磁珠对多个插入物进行纯化，然后放大纯化的多个插入物。 此外，该方法还包括对纯化和扩增的多个插入物进行测序。 在一些方面，多个插入物具有约800至约1,100个碱基对的长度。

    METHOD AND APPARATUS FOR FRAGMENTING DNA SEQUENCES
    7.
    发明申请
    METHOD AND APPARATUS FOR FRAGMENTING DNA SEQUENCES 有权
    用于克隆DNA序列的方法和装置

    公开(公告)号：US20120264228A1

    公开(公告)日：2012-10-18

    申请号：US13447606

    申请日：2012-04-16

    申请人： Dominique PONCELET ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

    发明人： Dominique PONCELET ,  Irina Panteleeva ,  Kazuo Ito ,  Didier Allaer

    IPC分类号： G01N1/44

    CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/301

    摘要： A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.

    摘要翻译： 将具有至少10000碱基对的起始大小的DNA序列片段化为平均大小小于或等于1300bp的片段的方法，其中将DNA序列置于溶液中，将包含DNA序列的溶液置于 将容器和容器放置在经受超声波作用的液浴中，使得超声波穿过液浴以激发容器和溶液以剪切DNA序列，并且其中超声波 频率落在28 kHz和80 kHz之间的范围内。