公开(公告)号：US5416025A

公开(公告)日：1995-05-16

申请号：US299330

申请日：1994-08-31

申请人： Jiri J. Krepinsky ,  Jacek Chociej ,  Gabor P. Kandel ,  Ka Sing Yeung

发明人： Jiri J. Krepinsky ,  Jacek Chociej ,  Gabor P. Kandel ,  Ka Sing Yeung

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/52 ,  G01N33/574 ,  G01N33/68 ,  C12Q1/26 ,  G01N33/53

CPC分类号： G01N33/52 ,  G01N33/57446

摘要： A method for detecting the presence of neoplasia or cancer of the colon or rectum, which method comprises obtaining a sample of colorectal mucus from the rectum of a patient; treating the sample with Schiff's reagent and screening for neoplasia or cancer of the colon or rectum based upon the coloration produced in the sample by the treatment. The method is rapid, simple, inexpensive and provides a screening test for colorectal cancer which does not give a high percentage of false positive and false negative results. A screening test kit is provided.

摘要翻译： 一种用于检测结肠或直肠瘤形成或癌症存在的方法，该方法包括从患者的直肠获得结肠直肠粘液样品; 用席夫试剂处理样品，并根据通过处理在样品中产生的着色来筛选结肠或直肠的肿瘤形成或癌症。 该方法快速，简单，便宜，为结肠直肠癌提供筛查试验，不会产生高百分比的假阳性和假阴性结果。 提供筛选测试试剂盒。

公开(公告)号：US06187591B1

公开(公告)日：2001-02-13

申请号：US09270103

申请日：1999-03-16

申请人： Jiri J Krepinsky ,  Jacek Chociej

发明人： Jiri J Krepinsky ,  Jacek Chociej

IPC分类号： G01N3368

CPC分类号： G01N33/57484 ,  G01N33/57419 ,  G01N33/57446 ,  Y10T436/200833

摘要： A method for detecting neoplasia or cancer of the colon or rectum comprising obtaining a sample of colorectal mucus from the rectum of a patient, and detecting the presence of a marker selected from the group consisting of long chain aliphatic aldehydes containing 12-20 carbon atoms, particularly CH3(CH2)14CHO and CH3(CH2)16CHO; and plasmalogen-bound precursors thereof. Preferably the method comprises treating the sample with Schiff's Reagent and detecting neoplasia or cancer of the colon or rectum based upon the coloration produced at about 560-590 nm in the sample by the treatment. The method does not require the step of adding an enzyme for detecting the disaccharide marker &bgr;-D-Gal(1-3)-D-GalNAc(&agr;1-Thr/Ser) and a saccharide marker containing D-galactose and/or 2-acetamido-2-deoxy-D-galactose.

摘要翻译： 一种用于检测结肠或直肠的肿瘤形成或癌症的方法，包括从患者的直肠获得结肠直肠粘液样品，并检测选自含有12-20个碳原子的长链脂族醛的标记物的存在， 特别是CH3（CH2）14CHO和CH3（CH2）16CHO; 和其与浆细胞结合的前体。 优选地，该方法包括用席夫氏试剂处理样品，并且基于通过处理在样品中在约560-590nm处产生的着色来检测结肠或直肠的肿瘤形成或癌症。 该方法不需要添加用于检测二糖标记β-D-Gal（1-3）-D-GalNAc（α1-Thr / Ser）的酶和含有D-半乳糖和/或2- 乙酰氨基-2-脱氧-D-半乳糖。