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公开(公告)号：US06187591B1
公开(公告)日：2001-02-13
申请号：US09270103
申请日：1999-03-16
申请人： Jiri J Krepinsky , Jacek Chociej
发明人： Jiri J Krepinsky , Jacek Chociej
IPC分类号： G01N3368
CPC分类号： G01N33/57484 , G01N33/57419 , G01N33/57446 , Y10T436/200833
摘要： A method for detecting neoplasia or cancer of the colon or rectum comprising obtaining a sample of colorectal mucus from the rectum of a patient, and detecting the presence of a marker selected from the group consisting of long chain aliphatic aldehydes containing 12-20 carbon atoms, particularly CH3(CH2)14CHO and CH3(CH2)16CHO; and plasmalogen-bound precursors thereof. Preferably the method comprises treating the sample with Schiff's Reagent and detecting neoplasia or cancer of the colon or rectum based upon the coloration produced at about 560-590 nm in the sample by the treatment. The method does not require the step of adding an enzyme for detecting the disaccharide marker &bgr;-D-Gal(1-3)-D-GalNAc(&agr;1-Thr/Ser) and a saccharide marker containing D-galactose and/or 2-acetamido-2-deoxy-D-galactose.
摘要翻译： 一种用于检测结肠或直肠的肿瘤形成或癌症的方法，包括从患者的直肠获得结肠直肠粘液样品，并检测选自含有12-20个碳原子的长链脂族醛的标记物的存在， 特别是CH3（CH2）14CHO和CH3（CH2）16CHO; 和其与浆细胞结合的前体。 优选地，该方法包括用席夫氏试剂处理样品，并且基于通过处理在样品中在约560-590nm处产生的着色来检测结肠或直肠的肿瘤形成或癌症。 该方法不需要添加用于检测二糖标记β-D-Gal（1-3）-D-GalNAc（α1-Thr / Ser）的酶和含有D-半乳糖和/或2- 乙酰氨基-2-脱氧-D-半乳糖。
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公开(公告)号：US5977328A
公开(公告)日：1999-11-02
申请号：US814885
申请日：1997-03-12
摘要： Methods for syntheses of polyvalent carbohydrate molecules by glycosylations of partially protected polysaccharides with a single glycosylating agent or a mixture of glycosylating agents (i.e., a combinatorial library) are disclosed. An alternate method utilizes a glycoside, the aglycon of which carries a halogen which can be under strong alkaline conditions linked with a partially protected polysaccharide by an ether linkage. The product of the latter reaction can be subjected to further glycosylation with a single glycosylating agent or a mixture of such agents (=library). The novel resulting polyvalent carbohydrate molecules may be used as antiinfective agents (antibacterial, antiparasital), both for prevention and treatment of diseases, and as agents either for preventing the formation of, or disrupting, biofilms.
摘要翻译： 公开了通过用单一糖基化剂或糖基化剂的混合物（即组合文库）通过部分保护的多糖的糖基化来合成多价碳水化合物分子的方法。 一种替代方法是使用糖苷，其糖苷配基携带卤素，其可以在与部分保护的多糖通过醚键连接的强碱性条件下。 后一反应的产物可以用单一糖基化剂或这些试剂的混合物（=文库）进一步糖基化。 所得到的多价碳水化合物分子可用作抗感染剂（抗细菌剂，抗天ital剂），用于预防和治疗疾病，以及作为用于防止生物膜形成或破坏的试剂。
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公开(公告)号：US5730985A
公开(公告)日：1998-03-24
申请号：US259004
申请日：1994-06-13
IPC分类号： A61K38/00 , A61P25/36 , C07F9/6561 , C07K16/44 , G01N33/94 , A61K39/385 , C07C69/76 , C07D451/12 , C07F9/02
CPC分类号： C07K16/44 , A61K39/0013 , C07F9/6561 , G01N33/946 , A61K2039/6081 , A61K2039/627 , A61K38/00
摘要： Methods are described for the rapid synthesis in satisfactory yield of methyl ecgonine phenylphosphonates as analogues of transition states for the hydrolysis of the benzoyl ester of an ecgonine derivative, namely cocaine, and their linking to carrier proteins, for the purpose of using them as immunogens. The resulting immunogens elicit the formation in experimental animals of antibodies able to promote the hydrolysis of cocaine. Both these catalytic anti-cocaine antibodies and the immunogens themselves are potentially useful for the treatment of individuals seeking to avoid the pharmacological effects of cocaine and in diagnostic applications.
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公开(公告)号：US06184368B2
公开(公告)日：2001-02-06
申请号：US09342164
申请日：1999-06-28
IPC分类号： C08B3108
摘要： Methods for syntheses of polyvalent carbohydrate molecules by glycosylations of partially protected polysaccharides with a single glycosylating agent or a mixture of glycosylating agents(i.e., a combinatorial library) are disclosed. An alternate method utilizes a glycoside, the aglycon of which carries a halogen which can be under strong alkaline conditions linked with a partially protected polysaccharide by an ether linkage. The product of the latter reaction can be subjected to further glycosylation with a single glycosylating agent or a mixture of such agents (=library). The novel resulting polyvalent carbohydrate molecules may be used as antiinfective agents (antibacterial, antiparasital), both for prevention and treatment of diseases, and as agents either for preventing the formation of, or disrupting, biofilms.
摘要翻译： 公开了通过用单一糖基化剂或糖基化剂的混合物（即组合文库）通过部分保护的多糖的糖基化来合成多价碳水化合物分子的方法。 一种替代方法是使用糖苷，其糖苷配基携带卤素，其可以在与部分保护的多糖通过醚键连接的强碱性条件下。 后一反应的产物可以用单一糖基化剂或这些试剂的混合物（=文库）进一步糖基化。 所得到的多价碳水化合物分子可用作抗感染剂（抗细菌剂，抗天ital剂），用于预防和治疗疾病，以及作为用于防止生物膜形成或破坏的试剂。
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公开(公告)号：US5616698A
公开(公告)日：1997-04-01
申请号：US179096
申请日：1994-01-10
IPC分类号： B01J19/00 , C07H3/04 , C07H3/06 , C07H15/18 , C40B40/12 , C40B50/08 , C40B60/14 , C07G3/00 , C07H1/00 , C07H15/04 , C08B37/00
CPC分类号： C07H15/18 , B01J19/0046 , C07H3/04 , C07H3/06 , B01J2219/00286 , B01J2219/00423 , B01J2219/00599 , B01J2219/00731 , C40B40/12 , C40B50/08 , C40B60/14
摘要： The present invention provides improved syntheses of oligosacchairdes. In accordance with preferred embodiments, anomeric specificity in such syntheses can be attained using polymer-supported liquid synthetic design with certain novel diether linkers. The present invention also provides novel strategies for capping imcompletely glycosylated hydroxyls.
摘要翻译： 本发明提供了改进的寡糖酯的合成。 根据优选实施方案，可以使用具有某些新型二醚连接体的聚合物支持的液体合成设计来获得这种合成中的端基异构体。 本发明还提供了不完全糖基化羟基封端的新策略。
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公开(公告)号：US5416025A
公开(公告)日：1995-05-16
申请号：US299330
申请日：1994-08-31
CPC分类号： G01N33/52 , G01N33/57446
摘要： A method for detecting the presence of neoplasia or cancer of the colon or rectum, which method comprises obtaining a sample of colorectal mucus from the rectum of a patient; treating the sample with Schiff's reagent and screening for neoplasia or cancer of the colon or rectum based upon the coloration produced in the sample by the treatment. The method is rapid, simple, inexpensive and provides a screening test for colorectal cancer which does not give a high percentage of false positive and false negative results. A screening test kit is provided.
摘要翻译： 一种用于检测结肠或直肠瘤形成或癌症存在的方法，该方法包括从患者的直肠获得结肠直肠粘液样品; 用席夫试剂处理样品，并根据通过处理在样品中产生的着色来筛选结肠或直肠的肿瘤形成或癌症。 该方法快速，简单，便宜，为结肠直肠癌提供筛查试验，不会产生高百分比的假阳性和假阴性结果。 提供筛选测试试剂盒。
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7.发明授权
公开(公告)号：US6017541A
公开(公告)日：2000-01-25
申请号：US906151
申请日：1997-08-05
IPC分类号： A61K38/00 , A61P25/36 , C07F9/6561 , C07K16/44 , G01N33/94 , A61K39/385 , C07K16/00
CPC分类号： C07K16/44 , A61K39/0013 , C07F9/6561 , G01N33/946 , A61K2039/6081 , A61K2039/627 , A61K38/00
摘要： Methods are described for the rapid synthesis in satisfactory yield of methyl ecgonine phenylphosphonates as analogues of transition states for the hydrolysis of the benzoyl ester of an ecgonine derivative, namely cocaine, and their linking to carrier proteins, for the purpose of using them as immunogens. The resulting immunogens elicit the formation in experimental animals of antibodies able to promote the hydrolysis of cocaine. Both these catalytic anti-cocaine antibodies and the immunogens themselves are potentially useful for the treatment of individuals seeking to avoid the pharmacological effects of cocaine and in diagnostic applications.
摘要翻译： 描述了为了以使用它们作为免疫原的目的，快速合成以令人满意的产率的甲基厄舌膦酸苯基膦酸酯作为类似物，用于水解伊万可因衍生物（即可卡因）的苯甲酰酯及其与载体蛋白的连接的过渡态的类似物。 所得到的免疫原引起实验动物中能够促进可卡因水解的抗体的形成。 这些催化抗可卡因抗体和免疫原本身都可能用于治疗寻求避免可卡因的药理作用和诊断应用的个体。
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公开(公告)号：US5278303A
公开(公告)日：1994-01-11
申请号：US898030
申请日：1992-06-12
摘要： This invention relates to the preparation of oligosaccharides, using polymer supported methodology. By this method, which offers anomeric control, oligosaccharides are produced very rapidly in comparison with known methodologies. Thus, there is disclosed a process for the preparation of oligosaccharides which comprisesa) forming a synthon of a saccharide and a monomethylether of polyethylene glycol or a derivative thereof, the synthon having a linkage between the oligosaccharide and the monomethylether of polyethylene glycol or a derivative thereof, which linkage can be severed under conditions that do not damage glycosidic or other bonds in a desired end product;b) subjecting the synthon to repeated additions of a suitable glycosylating agent to form a desired oligosaccharide-polyethylene glycol linked product;c) isolating the linked product as a solid;d) purifying this solid; ande) releasing the oligosaccharide from the polyethylene glycol.
摘要翻译： 本发明涉及使用聚合物支持的方法制备低聚糖。 通过这种提供端基异构体控制的方法，与已知方法相比，低聚糖产生非常快。 因此，公开了一种制备低聚糖的方法，其包括：a）形成糖类和聚乙二醇或其衍生物的单甲基醚的合成子，所述合成子在寡糖和聚乙二醇或其衍生物的单甲基醚之间具有连接 其可以在不损害所需最终产物中的糖苷或其它键的条件下切断该连接; b）使合成子重复加入合适的糖基化剂以形成所需的寡糖 - 聚乙二醇连接产物; c）以固体形式分离连接的产物; d）纯化该固体; 和e）从聚乙二醇中释放寡糖。
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