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1.发明申请Device and method for controlled electroporation and molecular delivery into cells and tissue 审中-公开用于受控电穿孔和分子传递到细胞和组织中的装置和方法公开(公告)号：US20050170510A1
公开(公告)日：2005-08-04
申请号：US11007661
申请日：2004-12-07
申请人： Yong Huang , James Borninski , Laura Mazzola
发明人： Yong Huang , James Borninski , Laura Mazzola
CPC分类号： C12M35/02 , C12N13/00 , C12N15/87 , C12N2510/00
摘要： In biology and biotechnology, electroporation is an important technique for introducing entities (DNA, RNAi, peptides, proteins, antibodies, genes, small molecules, nanoparticles, etc.) into cells. Applications range widely from genetic engineering to regenerative medicine to drug delivery. It has been demonstrated that the electrical currents flowing through cells can be used to monitor and control the process of electroporation for biological and artificial cells. In this application, a device and system are disclosed which allow precise monitoring and controlling electroporation of cells and cell layers, with examples shown using adherent cells grown on porous membranes.
摘要翻译： 在生物和生物技术中，电穿孔是将实体（DNA，RNAi，肽，蛋白质，抗体，基因，小分子，纳米粒子等）引入细胞的重要技术。 应用范围广泛，从遗传工程到再生医学到药物输送。 已经证明，流过细胞的电流可用于监测和控制生物和人造细胞的电穿孔过程。 在本申请中，公开了允许精确监测和控制细胞和细胞层电穿孔的装置和系统，其中使用在多孔膜上生长的贴壁细胞显示的实例。
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公开(公告)号：US06472859B1
公开(公告)日：2002-10-29
申请号：US09495241
申请日：2000-01-31
IPC分类号： G01R1900
CPC分类号： G01R31/025
摘要： An electrical ground detection circuit 10 having a first capacitor 18 and a second capacitor 20 which selectively receives a certain amount of voltage. The certain amount of voltage decreases by a certain amount during a period of time, as the capacitor 18 charges. The circuit 10 further includes a controller 16 which measures this period of time and which utilizes this measured period of time to determine whether an electrical assembly 12 is coupled to an electrically grounded member 14.
摘要翻译： 具有选择性地接收一定量电压的第一电容器18和第二电容器20的电接地检测电路10。 随着电容器18的充电，一定时间内电压的一定量减少一定量。 电路10还包括控制器16，该控制器16测量该时间周期，并利用该测量的时间段来确定电组件12是否耦合到电接地部件14。
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