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    Automated epifluorescence microscopy for detection of bacterial contamination in platelets
    1.
    发明授权
    Automated epifluorescence microscopy for detection of bacterial contamination in platelets 失效
    自动落射荧光显微镜检测血小板中的细菌污染

    公开(公告)号：US06803208B2

    公开(公告)日：2004-10-12

    申请号：US09916272

    申请日：2001-07-30

    申请人： Mark Seaver ,  James C. Crookston ,  Stephen J. Wagner

    发明人： Mark Seaver ,  James C. Crookston ,  Stephen J. Wagner

    IPC分类号： C12Q104

    CPC分类号： G01N33/80 ,  G01N33/56911

    摘要： A method for determining the presence of bacteria in a platelet or red blood cell sample is disclosed. The method includes the steps of: lysing a substantial portion of the platelets or red blood cells; staining the bacteria using a membrane permeable nucleic acid stain; filtering the sample using a membrane filter with a suitable pore size so that a material containing the stained bacteria is retained on the membrane filter; and analyzing the material retained on the membrane filter using epifluorescence microscopy and/or digital image acquisition and analysis to determine the presence of bacteria in the sample. The method allows the detection of bacterial contamination in platelets or red blood cells at clinically significant levels in a relatively short period of time.

    摘要翻译： 公开了一种确定血小板或红细胞样品中细菌存在的方法。 该方法包括以下步骤：裂解大部分血小板或红细胞; 使用膜渗透性核酸染色染色细菌; 使用具有适当孔径的膜过滤器过滤样品，使得含有染色细菌的材料保留在膜过滤器上; 并使用落射荧光显微镜和/或数字图像采集和分析分析保留在膜滤器上的材料，以确定样品中细菌的存在。 该方法允许在相当短的时间内以临床显着水平检测血小板或红细胞中的细菌污染。