公开(公告)号：US5434065A

公开(公告)日：1995-07-18

申请号：US58299

申请日：1993-05-06

申请人： Michael J. Mahan ,  John J. Mekalanos ,  James M. Slauch

发明人： Michael J. Mahan ,  John J. Mekalanos ,  James M. Slauch

IPC分类号： C07K14/255 ,  C12N15/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/09

CPC分类号： C12N15/1082 ,  C07K14/255 ,  C12N15/52 ,  C12N15/62 ,  C12N15/907 ,  C12Q1/6897 ,  C07K2319/00 ,  C07K2319/10 ,  C07K2319/80

摘要： A genetic system termed in vivo expression technology was devised that positively selects for microbial genes that are specifically induced when microbes infect their host. The method of this invention comprises complementing the growth of an auxotrophic or antibiotic sensitive microorganism by integrating an expression vector by way of homologous recombination into the auxotrophic or antibiotic sensitive microorganism's chromosome and inducing the expression of a synthetic operon which encodes transcripts, the expression of which are easily monitored both in vitro and in vivo.

摘要翻译： 设计了一种称为体内表达技术的遗传系统，可以积极选择当微生物感染宿主时特异性诱导的微生物基因。 本发明的方法包括通过将表达载体通过同源重组整合到营养缺陷型或抗生素敏感性微生物的染色体中并诱导编码转录物的合成操纵子的表达来补充营养缺陷型或抗生素敏感性微生物的生长，其表达 在体外和体内均易于监测。