公开(公告)号：US4259443A

公开(公告)日：1981-03-31

申请号：US47937

申请日：1979-06-12

申请人： James P. Danehy

发明人： James P. Danehy

IPC分类号： C07D307/62 ,  C07H7/033 ,  C07H15/04 ,  C12P7/58 ,  C12P7/60 ,  C12P17/04 ,  C12N9/04

CPC分类号： C07H15/04 ,  C07D307/62 ,  C07H7/033 ,  C12P17/04 ,  C12P7/58 ,  C12P7/60

摘要： A method of synthesizing vitamin C (ascorbic acid) directly from the hydrolysis products of lactose. Lactose, economically obtained from whey, undergoes hydrolysis with a warm aqueous slurry of lactase to produce D-galactose and D-glucose. Preparing the methyl glycosides of these two sugars protects a labile C-O linkage during the oxidation of the sugars to D-galacturonic acid and D-glucuronic acid. The mixture of these acids, after the removal of the methyl group through hydrolysis, undergoes reduction with gaseous hydrogen in the presence of an Adams catalyst or Raney nickel to produce a mixture of L-gulonic acid and L-galactonic acid. Removing the water from these acids forces their conversion into the corresponding lactones. Because of the applicable rate constants, adding water to the lactones does not result in their rapid reconversion to the acids. Accordingly, they can then undergo oxidation, in the presence of an enzyme obtained from pea seeds, to L-ascorbic acid.

摘要翻译： 从乳糖水解产物中直接合成维生素C（抗坏血酸）的方法。 从乳清经济地获得的乳糖用乳糖酶的温水性浆液进行水解以产生D-半乳糖和D-葡萄糖。 制备这两种糖的甲基糖苷可保护糖在D-半乳糖醛酸和D-葡萄糖醛酸氧化过程中不稳定的C-O键。 这些酸的混合物在通过水解除去甲基后，在亚当斯催化剂或阮内镍存在下用气态氢还原，生成L-古洛糖酸和L-半乳糖酸的混合物。 从这些酸中去除水会迫使其转化成相应的内酯。 由于适用的速率常数，向内酯中加入水不会导致其快速再转化为酸。 因此，它们可以在从豌豆种子获得的酶的存在下进行氧化，进行L-抗坏血酸。