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公开(公告)号：US09109264B2
公开(公告)日：2015-08-18
申请号：US12830574
申请日：2010-07-06
申请人： Jeffrey M. Linnen , Daniel P. Kolk , Janel M. Dockter , Damon K. Getman , Tadashi Yoshimura , Martha K. Ho-Sing-Loy , Reinhold B. Pollner , Leslie A. Stringfellow
发明人： Jeffrey M. Linnen , Daniel P. Kolk , Janel M. Dockter , Damon K. Getman , Tadashi Yoshimura , Martha K. Ho-Sing-Loy , Reinhold B. Pollner , Leslie A. Stringfellow
摘要： Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids.
摘要翻译： 用于检测病毒核酸的组合物，方法和试剂盒。 可以根据本发明检测的靶标包括HBV和/或HIV-1和/或HCV核酸。 特别描述的是可用作便于检测非常低水平的HBV核酸的杂交探针和扩增引物的寡核苷酸。
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公开(公告)号：US20110081645A1
公开(公告)日：2011-04-07
申请号：US12830574
申请日：2010-07-06
申请人： Jeffrey M. LINNEN , Daniel P. KOLK , Janel M. DOCKTER , Damon K. GETMAN , Tadashi YOSHIMURA , Martha K. HO-SING-LOY , Reinhold B. POLLNER , Leslie A. STRINGFELLOW
发明人： Jeffrey M. LINNEN , Daniel P. KOLK , Janel M. DOCKTER , Damon K. GETMAN , Tadashi YOSHIMURA , Martha K. HO-SING-LOY , Reinhold B. POLLNER , Leslie A. STRINGFELLOW
IPC分类号： C12Q1/70
摘要： Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids.
摘要翻译： 用于检测病毒核酸的组合物，方法和试剂盒。 可以根据本发明检测的靶标包括HBV和/或HIV-1和/或HCV核酸。 特别描述的是可用作便于检测非常低水平的HBV核酸的杂交探针和扩增引物的寡核苷酸。
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3.发明申请公开(公告)号：US20120003646A1
公开(公告)日：2012-01-05
申请号：US13173558
申请日：2011-06-30
申请人： Sunghae A. JOO , Janel M. DOCKTER
发明人： Sunghae A. JOO , Janel M. DOCKTER
CPC分类号： C12Q1/6816 , C12Q1/6844 , C12Q2545/101 , C12Q2537/165 , C12Q2545/114
摘要： Apparatus and method for detecting analyte in a reaction mixture comprising an internal control. The invention is illustrated using amplification and detection of nucleic acids, where the amplification reaction comprises an exogenous internal control. Magnitude of the detection signal serves as the variable for identifying invalid trials, identifying valid trials that are negative for analyte, and identifying trials that are positive for analyte. Detection of a signal indicating probe hybridization can be used for assigning the presence or absence of analyte nucleic acid in validated reactions using only a single detectable label, and without distinguishing the proportion of signal contributed by internal control probe binding, and analyte probe binding.
摘要翻译： 用于检测包含内部对照的反应混合物中的分析物的装置和方法。 使用扩增和检测核酸来说明本发明，其中扩增反应包括外源内部对照。 检测信号的大小用作识别无效试验的变量，识别分析物阴性的有效试验，并鉴定对分析物呈阳性的试验。 指示探针杂交的信号的检测可以用于仅使用单个可检测标记来在有效反应中分配分析物核酸的存在或不存在，并且不区分由内部对照探针结合贡献的信号的比例和分析物探针结合。
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公开(公告)号：US07785844B2
公开(公告)日：2010-08-31
申请号：US11872538
申请日：2007-10-15
申请人： Jeffrey M. Linnen , Daniel P. Kolk , Janel M. Dockter , Damon K. Getman , Tadashi Yoshimura , Martha K. Ho-Sing-Loy , Reinhold B. Pollner , Leslie A. Stringfellow
发明人： Jeffrey M. Linnen , Daniel P. Kolk , Janel M. Dockter , Damon K. Getman , Tadashi Yoshimura , Martha K. Ho-Sing-Loy , Reinhold B. Pollner , Leslie A. Stringfellow
摘要： Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids.
摘要翻译： 用于检测病毒核酸的组合物，方法和试剂盒。 可以根据本发明检测的靶标包括HBV和/或HIV-1和/或HCV核酸。 特别描述的是可用作便于检测非常低水平的HBV核酸的杂交探针和扩增引物的寡核苷酸。
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公开(公告)号：US09822397B2
公开(公告)日：2017-11-21
申请号：US13173558
申请日：2011-06-30
申请人： Sunghae A. Joo , Janel M. Dockter
发明人： Sunghae A. Joo , Janel M. Dockter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6844 , C12Q2545/101 , C12Q2537/165 , C12Q2545/114
摘要： Apparatus and method for detecting analyte in a reaction mixture comprising an internal control. The invention is illustrated using amplification and detection of nucleic acids, where the amplification reaction comprises an exogenous internal control. Magnitude of the detection signal serves as the variable for identifying invalid trials, identifying valid trials that are negative for analyte, and identifying trials that are positive for analyte. Detection of a signal indicating probe hybridization can be used for assigning the presence or absence of analyte nucleic acid in validated reactions using only a single detectable label, and without distinguishing the proportion of signal contributed by internal control probe binding, and analyte probe binding.
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