Plants with modified starch metabolism

    公开(公告)号:US10100324B2

    公开(公告)日:2018-10-16

    申请号:US12745237

    申请日:2008-11-27

    摘要: The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).

    Image authenticating methods
    3.
    发明授权
    Image authenticating methods 有权
    图像验证方法

    公开(公告)号:US07333632B2

    公开(公告)日:2008-02-19

    申请号:US10536315

    申请日:2003-11-24

    IPC分类号: G06K9/00

    CPC分类号: G08G1/054

    摘要: To authenticate images taken by image capturing elements (3) of offending vehicles, for example, when exceeding the authorized speed, provided elements are provided for allowing informative data on the offence to be supplied, such as the speed of the vehicle, the date, the time and the place of the offence and various processing methods are provided which enable, during exploitation of the images, detection of whether or not manipulations have been carried out on the images.

    摘要翻译: 为了验证图像捕获元件(3)拍摄的违规车辆的图像,例如,当超过授权速度时,提供了用于允许提供关于犯罪的信息的数据,例如车辆的速度,日期, 提供了攻击的时间和地点以及各种处理方法,在图像的利用期间,检测是否对图像进行了操作。

    Method and apparatus for treating silica granules using porous graphite crucible
    4.
    发明授权
    Method and apparatus for treating silica granules using porous graphite crucible 有权
    使用多孔石墨坩埚处理二氧化硅颗粒的方法和设备

    公开(公告)号:US06319550B1

    公开(公告)日:2001-11-20

    申请号:US09358817

    申请日:1999-07-22

    IPC分类号: B05D700

    摘要: Silica granules made up of mutually agglomerated silica soot particles are treated by placing the granules in a crucible inside a furnace which contains an inert gas mixed with a chlorine-containing compound and in which the temperature lies in the range 1000° C. to 1500° C. According to the invention, a crucible is used that is made of porous graphite firstly to increase the surface area of the crucible through which the chlorine-containing compound diffuses. This gives rise to faster exchange between the granules and the gas for given partial pressure of the chlorine-containing compound, thereby improving purification. Secondly, the difference between the chemical natures of the silicas and the crucible make the two materials inert relative to each other so that the granules do not adhere to the walls of the crucible, thereby making it possible to recover the granules without having to apply any mechanical operation that might contaminate them.

    摘要翻译: 将由相互团聚的二氧化硅烟灰颗粒构成的二氧化硅颗粒通过将颗粒放入含有与含氯化合物混合的惰性气体的炉内的坩埚中进行处理,其中温度在1000℃至1500℃的范围内 C.根据本发明,首先使用由多孔石墨制成的坩埚以增加含氯化合物通过其扩散的坩埚的表面积。 这导致颗粒和气体之间的交换更快,以使给定的含氯化合物分压,从而改进纯化。 其次,二氧化硅和坩埚的化学性质之间的差异使得两种材料相对于彼此是惰性的,使得颗粒不粘附到坩埚的壁上,从而使得可以回收颗粒而不必施加任何 机械操作可能会污染它们。

    PLANTS WITH MODIFIED STARCH METABOLISM
    5.
    发明申请
    PLANTS WITH MODIFIED STARCH METABOLISM 审中-公开
    具有改性淀粉代谢的植物

    公开(公告)号:US20110045127A1

    公开(公告)日:2011-02-24

    申请号:US12745237

    申请日:2008-11-27

    摘要: The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).

    摘要翻译: 本说明书提供了获得与对照植物相比具有增加的生产潜力的遗传修饰植物的方法,所述方法包括以下步骤:i)获得多个植物,其中至少一个在其基因组中包含异源多核苷酸,ii)鉴定 来自多个植物的植物相对于对照植物具有增加的生产潜力并且包含异源多核苷酸,以及iii)选择遗传修饰植物,其中所述多核苷酸包含可操作地连接到编码 修饰植物内源性淀粉磷酸化和/或淀粉降解的试剂。 在一些实施方案中,与对照植物相比,植物具有增加的内源糖基化酶或增加的消化率。 在一些具体实施方案中,内源性淀粉磷酸化和/或淀粉降解通过修饰选自以下的一种或多种酶的表达或活性来修饰:α-淀粉酶(EC 3.2.1.1),淀粉酶(EC 3.2。 1.2),葡糖淀粉酶(EC 3.2.1.3),淀粉磷酸化酶(EC2.4.1.1),糖基化酶(EC 3.1.33),蔗糖酶异麦芽糖酶(EC 3.2.10),淀粉麦芽糖酶(EC 2.4.1.25),麦芽糖酶 3.2.1.20),异淀粉酶和α-葡聚糖,水二激酶(GWD,EC 2.7.9.4)。

    Semicontinuous method of drawing fibers from preforms, the method including a preheating step
    6.
    发明授权
    Semicontinuous method of drawing fibers from preforms, the method including a preheating step 失效
    从预成型件拉伸纤维的半连续方法,该方法包括预热步骤

    公开(公告)号:US06209358B1

    公开(公告)日:2001-04-03

    申请号:US09234313

    申请日:1999-01-21

    IPC分类号: C03B3702

    摘要: In the fiber-drawing method, a first preform is lowered along a fiber-drawing axis through a fiber-drawing oven so as to be heated and drawn down into an optical fiber, and a second preform is lowered along the fiber-drawing axis through the fiber-drawing oven so as to be heated and drawn down in turn after the first preform has been withdrawn. While the first preform is being drawn down into a fiber, the second preform is heated in a preheating oven disposed adjacent to the fiber-drawing oven so as to raise the second preform to a temperature which is slightly below the fiber-drawing temperature, and the preheated second preform is transferred quickly onto the fiber-drawing axis after the first preform has been withdrawn therefrom. This method contributes to increasing the capacity for producing optical fibers.

    摘要翻译: 在纤维拉伸法中,第一预成型体沿着拉丝轴线通过纤维拉伸炉降低,以被加热并拉伸成光纤,并且第二预成型体沿着拉丝轴线通过 该纤维拉伸炉在第一预型件已经被取出之后依次被加热和拉下。 当第一预成型件被拉伸成纤维时,第二预型件在与拉丝炉邻近设置的预热炉中加热,以将第二预制件升高到略低于拉丝温度的温度,以及 在第一预制件从其中取出之后,将预热的第二预型件快速地转移到拉丝轴上。 该方法有助于增加制造光纤的能力。