公开(公告)号：US5244785A

公开(公告)日：1993-09-14

申请号：US649483

申请日：1991-02-01

申请人： Rueyming Loor ,  Jeff Shindelman ,  Pyare L. Khanna

发明人： Rueyming Loor ,  Jeff Shindelman ,  Pyare L. Khanna

IPC分类号： G01N33/535 ,  G01N33/542 ,  G01N33/58

CPC分类号： G01N33/581 ,  G01N33/542 ,  Y10S435/975

摘要： A method for detecting the presence of a high-molecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecule, wherein the ED complex retains measurable complementation activity such that active .beta.-galactosidase is formed in the presence of an enzyme acceptor (EA); (2) contacting the ED complex and EA in the presence of a sample suspected of containing a high-molecular-weight analyte that reacts specifically with the analyte-specific binding molecule; and (3) relating the presence of the analyte in the sample to the formation of active .beta.-galactosidase enzyme.

公开(公告)号：US07824879B2

公开(公告)日：2010-11-02

申请号：US12008394

申请日：2008-01-09

申请人： Shireen Khan ,  Suyue Qian ,  Jeff Shindelman ,  George E. Withers, III ,  Eileen Gee ,  William H. Chapman, Jr. ,  Greg Bennett ,  Thomas D. Schaal

发明人： Shireen Khan ,  Suyue Qian ,  Jeff Shindelman ,  George E. Withers, III ,  Eileen Gee ,  William H. Chapman, Jr. ,  Greg Bennett ,  Thomas D. Schaal

IPC分类号： C12Q1/60

CPC分类号： G01N33/526 ,  C12Q1/60 ,  G01N33/92

摘要： An assay device and method for determining the concentration of low density lipoprotein (LDL)-associated cholesterol (LDL-C) in a body-fluid sample, such as a blood sample is described.

摘要翻译： 描述了用于测定体液样品如血液样品中的低密度脂蛋白（LDL）相关胆固醇（LDL-C）的浓度的测定装置和方法。

公开(公告)号：US20080166745A1

公开(公告)日：2008-07-10

申请号：US12008394

申请日：2008-01-09

申请人： Shireen Khan ,  Suyue Qian ,  Jeff Shindelman ,  George E. Withers ,  Eileen Gee ,  William H. Chapman ,  Greg Bennett ,  Thomas D. Schaal

发明人： Shireen Khan ,  Suyue Qian ,  Jeff Shindelman ,  George E. Withers ,  Eileen Gee ,  William H. Chapman ,  Greg Bennett ,  Thomas D. Schaal

IPC分类号： C12Q1/60 ,  C12M1/34

CPC分类号： G01N33/526 ,  C12Q1/60 ,  G01N33/92

摘要： An assay device and method for determining the concentration of low density lipoprotein (LDL)-associated cholesterol (LDL-C) in a body-fluid sample, such as a blood sample is described.

摘要翻译： 描述了用于测定体液样品如血液样品中的低密度脂蛋白（LDL）相关胆固醇（LDL-C）的浓度的测定装置和方法。