公开(公告)号：US20130084652A1

公开(公告)日：2013-04-04

申请号：US13641476

申请日：2011-05-13

Applicant: Nir Shapir ,  Michael Salvati ,  Jeff Todtleben ,  Hashem Akhavan-Tafti ,  Richard S. Handley

Inventor： Nir Shapir ,  Michael Salvati ,  Jeff Todtleben ,  Hashem Akhavan-Tafti ,  Richard S. Handley

IPC: G01N21/76

CPC classification number: G01N21/76 ,  C12Q1/28 ,  C12Q1/66 ,  G01N33/5306 ,  G01N33/582

Abstract: Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. Non-specific signal generated by singlet oxygen not in proximity is reduced or suppressed using a singlet oxygen quencher (SOQ). The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Use of Noise Modulation Agents significantly improves signal-to-noise ratios and assay sensitivity. Compositions and kits are also disclosed.

Abstract translation: 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 使用单线态氧猝灭剂（SOQ）降低或抑制不接近的单线态氧产生的非特异性信号。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 使用噪声调制剂可显着提高信噪比和测定灵敏度。 还公开了组合物和试剂盒。

公开(公告)号：US20050136542A1

公开(公告)日：2005-06-23

申请号：US10741403

申请日：2003-12-19

Applicant: Jeff Todtleben ,  Stephen Chan

Inventor： Jeff Todtleben ,  Stephen Chan

IPC: G01N1/00 ,  G01N31/00 ,  G01N33/68 ,  G01N33/96

CPC classification number: G01N33/96 ,  G01N33/6887 ,  G01N2333/4712 ,  Y10T436/105831

Abstract: Stabilizing liquid reference solutions containing basic polypeptide analytes, amino acids with basic side chains and stabilizing protein are provided for use as calibrators and controls for immunoassays.

Abstract translation: 提供含有碱性多肽分析物，具有碱性侧链和稳定蛋白质的氨基酸的稳定液体参考溶液用作免疫测定的校准物和对照。