公开(公告)号：US20060160177A1

公开(公告)日：2006-07-20

申请号：US10967088

申请日：2004-10-15

申请人： Jens Okkels ,  Anne Jensen ,  Bart van den Hazel

发明人： Jens Okkels ,  Anne Jensen ,  Bart van den Hazel

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12P19/34 ,  C07K9/00 ,  C07K14/47 ,  C12N5/06 ,  C12N1/18

CPC分类号： A61K38/24 ,  C12P21/005

摘要： Glycosylated polypeptides comprising the primary structure NH2-X-Pp-COOH, wherein X is a peptide addition comprising or contributing to a glycosylation site, and Pp is a polypeptide of interest or comprising the primary structure NH2-Px-X-Py-COOH, wherein Px is an N-terminal part of a polypeptide Pp of interest, Py is a C-terminal part of said polypeptide Pp, and X is a peptide addition comprising or contributing to a glycosylation site are provided. The glycosylated polypeptides possess improved properties as compared to the polypeptide of interest.

摘要翻译： 包含一级结构NH 2 -X-Pp-COOH的糖基化多肽，其中X是包含或有助于糖基化位点的肽加成物，Pp是感兴趣的多肽或包含一级结构NH 其中P 是多肽Pp的N末端部分 感兴趣的是所述多肽Pp的C末端部分，并且X是包含或有助于糖基化位点的肽加成物。 与感兴趣的多肽相比，糖基化多肽具有改善的性质。

公开(公告)号：US20050222389A1

公开(公告)日：2005-10-06

申请号：US10756813

申请日：2004-01-12

申请人： Torben Halkier ,  Anders Pedersen ,  Jens Okkels ,  Kim Anderson

发明人： Torben Halkier ,  Anders Pedersen ,  Jens Okkels ,  Kim Anderson

IPC分类号： C07H21/04 ,  C07K1/107 ,  C07K14/31 ,  C07K14/47 ,  C07K19/00 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： C12N15/1058 ,  C07K1/107 ,  C07K14/31 ,  C07K19/00 ,  C12N15/1034

摘要： Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.

摘要翻译： 公开了产生具有改变的免疫原性或改善的稳定性的多肽的方法。 所述方法包括：a）表达编码感兴趣多肽的核苷酸序列的多样化群体，b）筛选功能，免疫原性和/或稳定性在步骤a）中表达的多肽，c）选择具有改变的免疫原性和/或增加稳定性的功能性多肽 ，例如 与目的多肽相比具有功能的体内半衰期，和d）任选地将编码步骤c）中选择的多肽的核苷酸序列经历步骤a）-c）的一个或多个重复循环。 在另一步骤中，步骤a）或c）的表达多肽可以与至少一个非多肽部分缀合。

公开(公告)号：US20050124066A9

公开(公告)日：2005-06-09

申请号：US10738669

申请日：2003-12-17

申请人： Jens Okkels

发明人： Jens Okkels

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N9/20 ,  C12N15/10 ,  C12P21/02 ,  C12R1/865 ,  C12N15/85 ,  C12N15/74

CPC分类号： C12N9/20 ,  C12N15/1027

摘要： The present invention relates to a method for preparing positive polypeptide variants by shuffling different nucleotide sequences of homologous DNA sequences by in vivo recombination comprising the steps of (a) forming at least one circular plasmid comprising a DNA sequence encoding a polypeptide, (b) opening said circular plasmid(s) within the DNA sequence(s) encoding the polypeptide(s), (c) preparing at least one DNA fragment comprising a DNA sequence homologous to at least a part of the polypeptide coding region on at least one of the circular plasmid(s), (d) introducing at least one of said opened plasmid(s), together with at least one of said homologous DNA fragment(s) covering full-length DNA sequences encoding said polypeptide(s) or parts thereof, into a recombination host cell, (e) cultivating said recombination host cell, and (f) screening for positive polypeptide variants.

摘要翻译： 本发明涉及通过体内重组来混合同源DNA序列的不同核苷酸序列来制备阳性多肽变体的方法，包括以下步骤：（a）形成至少一个环状质粒，其包含编码多肽的DNA序列，（b）开放 在编码多肽的DNA序列内的所述循环质粒，（c）制备至少一个DNA片段，所述至少一个DNA片段包含与至少一个所述多肽编码区的至少一部分同源的DNA序列 循环质粒，（d）将至少一个所述打开的质粒与至少一个所述同源DNA片段一起覆盖编码所述多肽或其部分的全长DNA序列， （e）培养所述重组宿主细胞，和（f）筛选阳性多肽变体。

公开(公告)号：US20050048649A1

公开(公告)日：2005-03-03

申请号：US10738669

申请日：2004-11-02

申请人： Jens Okkels

发明人： Jens Okkels

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N9/20 ,  C12N15/10 ,  C12P21/02 ,  C12R1/865 ,  C12N15/85 ,  C12N15/74

CPC分类号： C12N9/20 ,  C12N15/1027

摘要： The present invention relates to a method for preparing positive polypeptide variants by shuffling different nucleotide sequences of homologous DNA sequences by in vivo recombination comprising the steps of (a) forming at least one circular plasmid comprising a DNA sequence encoding a polypeptide, (b) opening said circular plasmid(s) within the DNA sequence(s) encoding the polypeptide(s), (c) preparing at least one DNA fragment comprising a DNA sequence homologous to at least a part of the polypeptide coding region on at least one of the circular plasmid(s), (d) introducing at least one of said opened plasmid(s), together with at least one of said homologous DNA fragment(s) covering full-length DNA sequences encoding said polypeptide(s) or parts thereof, into a recombination host cell, (e) cultivating said recombination host cell, and (f) screening for positive polypeptide variants.