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    Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
    5.
    发明授权
    Universal linker compositions for the release or transfer of chemical agents from a polynucleotide 有权
    用于从多核苷酸释放或转移化学试剂的通用接头组合物

    公开(公告)号：US07745614B2

    公开(公告)日：2010-06-29

    申请号：US11218961

    申请日：2005-09-01

    申请人： Eric Todd Kool ,  Hiroshi Abe

    发明人： Eric Todd Kool ,  Hiroshi Abe

    IPC分类号： C07H21/02 ,  C07H19/04 ,  C12Q1/68 ,  C07H21/00 ,  C07F9/00

    CPC分类号： C07F9/2429 ,  C07F9/08 ,  C07F9/2408 ,  Y10S977/792

    摘要： A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when the two nucleic acids are hybridized near one another, resulting in release of the leaving group, and creation of a functional change. The linker can be designed to destabilize the ligation product without slowing the rate of reaction. This lowers product inhibition, and the target DNA or RNA can become a catalyst for isothermally generating multiple signals for detection. This enhanced signal is demonstrated in solution experiments and in solid supported assays. The universal linkers of the present invention are simple and inexpensive to prepare, and can be appended to any polynucleotide in automated steps on a standard DNA synthesizer.

    摘要翻译： 提供通用接头结构，其中功能基团和​​活化离去基团置于系链上，允许在任何核酸序列末端放置亲电子试剂。 当两个核酸彼此相互杂交时，系链上的亲电子体可以与携带亲核试剂的第二核酸反应，导致离去基团的释放和功能变化的产生。 连接体可以被设计成使连接产物不稳定而不减慢反应速率。 这降低了产物抑制，目标DNA或RNA可以成为用于等温生成多个用于检测的信号的催化剂。 在溶液实验和固体支持的测定中证明了这种增强的信号。 本发明的通用接头是简单且廉价的制备，并且可以在标准DNA合成仪上以自动步骤附加到任何多核苷酸。

    Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
    6.
    发明申请
    Universal linker compositions for the release or transfer of chemical agents from a polynucleotide 有权
    用于从多核苷酸释放或转移化学试剂的通用接头组合物

    公开(公告)号：US20060199192A1

    公开(公告)日：2006-09-07

    申请号：US11218961

    申请日：2005-09-01

    申请人： Eric Kool ,  Hiroshi Abe

    发明人： Eric Kool ,  Hiroshi Abe

    IPC分类号： C12Q1/68 ,  C07F9/02 ,  C07H19/04 ,  A61K48/00

    CPC分类号： C07F9/2429 ,  C07F9/08 ,  C07F9/2408 ,  Y10S977/792

    摘要： A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when the two nucleic acids are hybridized near one another, resulting in release of the leaving group, and creation of a functional change. The linker can be designed to destabilize the ligation product without slowing the rate of reaction. This lowers product inhibition, and the target DNA or RNA can become a catalyst for isothermally generating multiple signals for detection. This enhanced signal is demonstrated in solution experiments and in solid supported assays. The universal linkers of the present invention are simple and inexpensive to prepare, and can be appended to any polynucleotide in automated steps on a standard DNA synthesizer.

    摘要翻译： 提供通用接头结构，其中功能基团和​​活化离去基团置于系链上，允许在任何核酸序列末端放置亲电子试剂。 当两个核酸彼此相互杂交时，系链上的亲电子体可以与携带亲核试剂的第二核酸反应，导致离去基团的释放和功能变化的产生。 连接体可以被设计成使连接产物不稳定而不减慢反应速率。 这降低了产物抑制，目标DNA或RNA可以成为用于等温生成多个用于检测的信号的催化剂。 在溶液实验和固体支持的测定中证明了这种增强的信号。 本发明的通用接头是简单且廉价的制备，并且可以在标准DNA合成仪上以自动步骤附加到任何多核苷酸。

    Dipentaerythritol and pentaerythritol phosphite
    8.
    发明授权
    Dipentaerythritol and pentaerythritol phosphite 失效
    二季戊四醇和季戊四醇亚磷酸酯

    公开(公告)号：US3928505A

    公开(公告)日：1975-12-23

    申请号：US51525474

    申请日：1974-10-16

    申请人： BORG WARNER

    发明人： KLICKER JAMES D ,  COHEN MURRAY S

    IPC分类号： C07F9/08 ,  C07F9/6574 ,  C08K5/524 ,  C07F9/15

    CPC分类号： C07F9/08 ,  C07F9/6574 ,  C08K5/524

    摘要： By reacting a mxiture of pentaerythritol and dipentaerythritol with a trihydrocarbyl or trihalohydrocarbyl phosphite, preferably triphenyl phosphite there is obtained pentaerythritoldipentaerythritol phosphite as a relatively low melting solid. In contrast to the use of only pentaerythritol or dipentaerythritol the product can be readily worked as a liquid above 110*C., e.g., 150*-160*C. Furthermore, the performance and physical properties of this product are not the same as that of an equal molar mixture of pure pentaerythritol phosphite and dipentaerythritol phosphite.

    摘要翻译： 通过使季戊四醇和二季戊四醇与三烃基或三卤代烃基亚磷酸酯反应，优选亚磷酸三苯酯，得到季戊四醇 - 二季戊四醇亚磷酸酯作为相对低熔点的固体。 与仅使用季戊四醇或二季戊四醇相反，该产品可以容易地作为高于110℃，例如150-116℃的液体加工。此外，该产品的性能和物理性质与该产品的性能和物理性质不同 纯的季戊四醇亚磷酸酯和二季戊四醇亚磷酸酯的等摩尔混合物。

    Preparation of esters of phosphorus acids
    9.
    发明授权
    Preparation of esters of phosphorus acids 失效
    磷酸酯的制备

    公开(公告)号：US3790649A

    公开(公告)日：1974-02-05

    申请号：US3790649D

    申请日：1971-06-24

    申请人： SCHUMACHER I ,  BAKER J

    发明人： SCHUMACHER I ,  BAKER J

    IPC分类号： C07F9/08 ,  C07F9/16

    CPC分类号： C07F9/08 ,  C07F9/16

    摘要： HALOGEN-CONTAINING ESTERS OF PHOSPHORUS ACIDS ARE PREPARED BY AN IMPROVED PROCESS WHEREBY THIOL- OR HYDROXYLCONTAINING ORGANIC MATERIALS AND PHOSPHORUS HALIDES ARE REACTED AT SPECIFIED TEMPERATURES IN THE PRESENCE OF A DISTILLATION RESIDUE, PRODUCED BY DISTILLING (A) THE PRODUCT DERIVED FROM A REACTION MIXTURE OF A THIOL- OR HYDROXYLCONTAINING ORGANIC MATERIAL, A PHOSPHORUS HALIDE AND A CATALYST MATERIAL SELECTED FROM AMIDE, PHOSPHORUS AMIDE, UREA AND AMMONIUM SALT COMPOUNDS CONTAINING A NITROGEN ATOM CAPABLE OF FORMING A COMPLEX WITH A PHOSPHORUS MOIETY OR (B) THE PRODUCT DERIVED FROM SUCH REACTION MIXTURE IN WHICH SAID DISTILLATION RESIDUE IS EMPLOYED AS THE CATALYST MATERIAL, THEREBY PROVIDING HIGH YIELDS OF SUBSTANTIALLY PURE ESTERS AND ALLOWING PREPARATION OF SELECTED HALOGEN-CONTAINING MONO- AND DI-ESTERS OF PHOSPHORUS ESTERS HAVING SUBSTANTIALLY NO SIDE REACTANT CONTAMINATION. THE PHOSPHORUS ESTERS ARE USEFUL AS INTERMEDIATES IN THE PREPARATION OF PLASTICIZERS, OIL ADDITIVES AND FUNCTIONAL FLUIDS.