Steep solvent gradient NMR analysis method
    5.
    发明授权
    Steep solvent gradient NMR analysis method 失效
    陡溶剂梯度NMR分析方法

    公开(公告)号:US06700379B2

    公开(公告)日:2004-03-02

    申请号:US10006503

    申请日:2001-12-03

    IPC分类号: G01V300

    摘要: An NMR method of analyzing an analyte comprises feeding an analyte sample fluid to an NMR flow cell. The NMR flow cell comprises an RF microcoil operably associated with an enlarged containment region. The mobile phase of the analyte sample flowing through the NMR flow cell has a solvent gradient greater than 10% per minute. The analyte sample fluid can be fed to the NMR flow cell from an analyte extraction chamber, e.g., operative to perform liquid chromatography, capillary electrophoresis, or the like, especially a capillary-based analyte extraction chamber integrated in an NMR probe with the NMR flow cell. A sample volume is held in the NMR flow cell for equilibration less than 1 hour, preferably less than 30 minutes prior to actuating NMR analysis of the observe volume in the microcoil.

    摘要翻译: 分析分析物的NMR方法包括将分析物样品流体进料到NMR流动池。 NMR流通池包括可操作地与扩大的容纳区域相关联的RF微线圈。 流过NMR流通池的分析物样品的流动相具有大于10%/分钟的溶剂梯度。 分析物样品流体可以从分析物提取室进料至NMR流动池,例如,可操作以进行液相色谱,毛细管电泳等,特别是集成在具有NMR流量的NMR探针中的基于毛细管的分析物提取室 细胞。 样品体积保存在NMR流通池中,用于平衡小于1小时,优选小于30分钟,然后在微量线圈上观察体积的NMR分析。