公开(公告)号：US20110301058A1

公开(公告)日：2011-12-08

申请号：US13156250

申请日：2011-06-08

申请人： Ji-Yen Cheng ,  Joel Chia-Yeh Chang ,  Ching-Wen Yang

发明人： Ji-Yen Cheng ,  Joel Chia-Yeh Chang ,  Ching-Wen Yang

IPC分类号： C40B30/04 ,  C40B99/00 ,  C40B60/12

CPC分类号： G01N33/54366 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/086

摘要： A novel approach for fabricating Monolithic Internal micro Pillars (MIPi) made of SU-8 photoresist is described. A microfluidic chip with the internal pillars (a MIPi chip) was used for cell capturing study. The surface of MIPi was coated with specific antibody and then used for capturing cells by affinity binding. An antibody, anti-EGFR, which has high affinity to lung cancer cells, CL1-5, was coated on the micro pillars. The coated MIPi chip specifically captured the cancer cells that were pumped through the MIPi chip. Simulation and experiment was carried out to compare the effect of different geometry of the micro pillars on the cell capturing rate.

摘要翻译： 描述了一种用于制造由SU-8光致抗蚀剂制成的单片内微型支柱（MIPi）的新方法。 使用具有内部柱的微流控芯片（MIPi芯片）用于细胞捕获研究。 用特异性抗体包被MIPi的表面，然后用于通过亲和结合捕获细胞。 将对肺癌细胞CL1-5具有高亲和力的抗体，抗EGFR，涂覆在微柱上。 涂覆的MIPi芯片专门捕获通过MIPi芯片泵送的癌细胞。 进行模拟和实验，比较微柱不同几何对细胞捕获率的影响。