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公开(公告)号：US20070087353A1
公开(公告)日：2007-04-19
申请号：US11249449
申请日：2005-10-14
申请人： Ji-Yen Cheng , Cheng-Wey Wei
发明人： Ji-Yen Cheng , Cheng-Wey Wei
CPC分类号： B01L3/502707 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2400/0487
摘要： The microarray biochemical reaction device of this invention integrates microfluidic trenches with a microarray to form a serpentine microchannel passing through all DNA probes provided in the microarray. A sample solution is introduced into the microchannel and scrambled into discrete plugs to induce droplet mixing. The discrete plugs are then shuttled through the entire microchannel (shuttle hybridization), sweeping over DNA probes to perform hybridization. Using chaotic mixing of droplets, the hybridization efficiency is enhanced and reaction time for hybridization is shortened. During shuttling, the plugs are thoroughly mixed by the natural re-circulating flows. Method for the preparation of the microarray biochemical reaction device is also disclosed.
摘要翻译： 本发明的微阵列生物化学反应装置将微流体沟槽与微阵列整合，以形成穿过微阵列中提供的所有DNA探针的蛇形微通道。 将样品溶液引入微通道并加扰成离散的塞子以诱导液滴混合。 然后将离散的塞子穿过整个微通道（穿梭杂交），扫过DNA探针进行杂交。 通过混合液滴混合，杂交效率提高，杂交反应时间缩短。 在穿梭期间，塞子被自然的循环流充分混合。 还公开了用于制备微阵列生化反应装置的方法。
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公开(公告)号：US07691624B2
公开(公告)日：2010-04-06
申请号：US11582867
申请日：2006-10-18
申请人： Ta-Chau Chang , Cheng-Chung Chang , Chi-Chih Kang , Ji-Yen Cheng
发明人： Ta-Chau Chang , Cheng-Chung Chang , Chi-Chih Kang , Ji-Yen Cheng
CPC分类号： G01N21/6428
摘要： This invention relates to a device for detecting cancer cells. The device includes a light source for generating light (e.g., a light emitting diode), a first optical filter selected from the group consisting of a band pass filter and a long pass filter, a second optical filter (i.e., a band pass filter) disposed between the light source and the first optical filter, and a sample receiver for receiving a sample. The sample receiver is disposed between the first and second optical filters. The first optical filter, the sample receiver, and the second optical filter are aligned so that light emitted from the light source passes sequentially through the second optical filter, the sample receiver, and the first optical filter.
摘要翻译： 本发明涉及一种用于检测癌细胞的装置。 该装置包括用于产生光的光源（例如，发光二极管），从由带通滤波器和长通滤波器组成的组中选择的第一滤光器，第二滤光器（即，带通滤光器） 设置在光源和第一滤光器之间，以及用于接收样品的样品接收器。 样品接收器设置在第一和第二滤光器之间。 对准第一滤光器，样品接收器和第二滤光器，使得从光源发射的光顺序地通过第二滤光器，样品接收器和第一滤光器。
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公开(公告)号：US20050101006A1
公开(公告)日：2005-05-12
申请号：US10705698
申请日：2003-11-08
申请人： Ji-Yen Cheng , Cheng-Wey Wei
发明人： Ji-Yen Cheng , Cheng-Wey Wei
CPC分类号： B81C1/00492 , B01L3/502707 , B01L2200/12 , B01L2300/165 , B05D1/185
摘要： The present invention discloses a method for preparation and surface modification of plastic microfluidic chip is disclosed. A microfluidic chip is first prepared by micromachining using a laser scriber on a plastic substrate, such as a PMMA substrate, to have a desired trench width or trench widths and desired aspect ratio. Surface modification is obtained after a thermal annealing treatment, followed by a chemical modification process. During the chemical modification diverse functional groups such as perfluoroalkyl (—CnF2n+2), amino (—NH2) or sulfhydryl (—SH) are introduced to perform surface passivation or further biomolecules immobilization. A plastic microfluidic chip with modified surface characters is thus obtained. The present invention also discloses plastic microfluidic chips so prepared.
摘要翻译： 本发明公开了一种塑料微流控芯片的制备和表面改性方法。 首先通过使用激光划刻器在诸如PMMA衬底的塑料衬底上的微加工来制备微流控芯片，以具有期望的沟槽宽度或沟槽宽度和期望的纵横比。 在热退火处理后进行表面改性，接着进行化学修饰处理。 在化学修饰期间，各种官能团如全氟烷基（-C≡N2 N 2n + 2），氨基（-NH 2/2）或巯基（ -SH）进行表面钝化或进一步的生物分子固定。 从而获得具有改进的表面特性的塑料微流体芯片。 本发明还公开了如此制备的塑料微流体芯片。
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公开(公告)号：US20110301058A1
公开(公告)日：2011-12-08
申请号：US13156250
申请日：2011-06-08
CPC分类号： G01N33/54366 , B01L3/5027 , B01L2300/0636 , B01L2300/0877 , B01L2400/086
摘要： A novel approach for fabricating Monolithic Internal micro Pillars (MIPi) made of SU-8 photoresist is described. A microfluidic chip with the internal pillars (a MIPi chip) was used for cell capturing study. The surface of MIPi was coated with specific antibody and then used for capturing cells by affinity binding. An antibody, anti-EGFR, which has high affinity to lung cancer cells, CL1-5, was coated on the micro pillars. The coated MIPi chip specifically captured the cancer cells that were pumped through the MIPi chip. Simulation and experiment was carried out to compare the effect of different geometry of the micro pillars on the cell capturing rate.
摘要翻译： 描述了一种用于制造由SU-8光致抗蚀剂制成的单片内微型支柱（MIPi）的新方法。 使用具有内部柱的微流控芯片（MIPi芯片）用于细胞捕获研究。 用特异性抗体包被MIPi的表面，然后用于通过亲和结合捕获细胞。 将对肺癌细胞CL1-5具有高亲和力的抗体，抗EGFR，涂覆在微柱上。 涂覆的MIPi芯片专门捕获通过MIPi芯片泵送的癌细胞。 进行模拟和实验，比较微柱不同几何对细胞捕获率的影响。
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公开(公告)号：US20090017528A1
公开(公告)日：2009-01-15
申请号：US11777265
申请日：2007-07-12
申请人： Pei-Tai Chen , Ji-Yen Cheng
发明人： Pei-Tai Chen , Ji-Yen Cheng
IPC分类号： C12M1/34
CPC分类号： B01F13/0071 , B01F11/0283 , B01L3/5085 , B01L3/5088
摘要： The present invention relates to a hybridization device coupled by means of vibration to shorten the time needed for hybridization. This hybridization device of the present invention comprises an oscillation generating unit; a spacer attached to the top of the oscillation generating unit, which wraps around to form a tank, and the tank is loaded with a transmission solution; a loading plate having a loading face was paved with at least one nucleic acid probe, and the flip side of the loading face is a transmission face, which covers on top of the spacer to form a closed space, and is attached with the transmission solution. The vibration generated by the oscillation generating unit can be propagated to the loading plate through the transmission solution to promote the effective mixing of the nucleic acid to be analyzed and the probe, further to shorten the time needed for nucleic acid hybridization and to increase the detection sensitivity.
摘要翻译： 本发明涉及通过振动耦合的杂交装置，以缩短杂交所需的时间。 本发明的杂交装置包括振荡发生单元; 附接到振荡发生单元的顶部的间隔件，其围绕形成罐，并且罐装载有传输溶液; 具有装载面的装载板铺设有至少一个核酸探针，并且装载面的反面是透明面，其覆盖在间隔物的顶部上以形成封闭空间，并且附着有透射溶液 。 由振荡产生单元产生的振动可以通过透射溶液传播到装载板，以促进要分析的核酸和探针的有效混合，进一步缩短核酸杂交所需的时间和增加检测 灵敏度。
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公开(公告)号：US20070098233A1
公开(公告)日：2007-05-03
申请号：US11582867
申请日：2006-10-18
申请人： Ta-Chau Chang , Cheng-Chung Chang , Chi-Chih Kang , Ji-Yen Cheng
发明人： Ta-Chau Chang , Cheng-Chung Chang , Chi-Chih Kang , Ji-Yen Cheng
CPC分类号： G01N21/6428
摘要： This invention relates to a device for detecting cancer cells. The device includes a light source for generating light (e.g., a light emitting diode), a first optical filter selected from the group consisting of a band pass filter and a long pass filter, a second optical filter (i.e., a band pass filter) disposed between the light source and the first optical filter, and a sample receiver for receiving a sample. The sample receiver is disposed between the first and second optical filters. The first optical filter, the sample receiver, and the second optical filter are aligned so that light emitted from the light source passes sequentially through the second optical filter, the sample receiver, and the first optical filter.
摘要翻译： 本发明涉及一种用于检测癌细胞的装置。 该装置包括用于产生光的光源（例如，发光二极管），从由带通滤波器和长通滤波器组成的组中选择的第一滤光器，第二滤光器（即，带通滤光器） 设置在光源和第一滤光器之间，以及用于接收样品的样品接收器。 样品接收器设置在第一和第二滤光器之间。 对准第一滤光器，样品接收器和第二滤光器，使得从光源发射的光顺序地通过第二滤光器，样品接收器和第一滤光器。
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公开(公告)号：US06867050B2
公开(公告)日：2005-03-15
申请号：US09923464
申请日：2001-08-06
申请人： Konan Peck , Ji-Yen Cheng
发明人： Konan Peck , Ji-Yen Cheng
CPC分类号： B01J19/0046 , B01J2219/00274 , Y10T436/2575
摘要： Apparatuses and methods are described for parallel oligonucleotide synthesis of hundreds of different sequences and lengths at a time. Standard phosphoramidite chemistry is employed. The syntheses take place in a reaction plate compatible with the industrial standard microplate format to allow the use of readily available automated instruments for subsequent processing. Key parameters in reducing synthesis volume in small reaction wells are discussed. This invention provides solutions to the difficulties of low volume, high number synthetic reactions.
摘要翻译： 描述了一次并行寡核苷酸合成数百种不同序列和长度的装置和方法。 使用标准亚磷酰胺化学。 合成在与工业标准微板格式兼容的反应板中进行，以允许使用容易获得的自动化仪器进行后续处理。 讨论了在小反应井中降低合成体积的关键参数。 本发明提供了低容量，高数量的合成反应的困难的解决方案。
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