公开(公告)号：US20080207509A1

公开(公告)日：2008-08-28

申请号：US11917057

申请日：2006-06-08

申请人： Robbie Woodman ,  Johannes Tsung-Hau Yeh ,  Sophie Laurenson ,  Paul Ko Ferrigno

发明人： Robbie Woodman ,  Johannes Tsung-Hau Yeh ,  Sophie Laurenson ,  Paul Ko Ferrigno

IPC分类号： A61K38/00 ,  C07K16/00 ,  C07H21/04 ,  G01N33/53

CPC分类号： C07K14/8139 ,  C07K7/06 ,  C12N15/1044

摘要： The present invention relates to the use of Stefin A as a scaffold protein for the display of inserted peptides, particularly wherein the Stefin A is a human Stefin A. Several mutations are advantageously made in the wild type stefin A sequence to improve it as a scaffold; preferably the Stefin A comprises a heterologous peptide insertion at the Leu 73 site. Furthermore, preferably the scaffold protein comprises a V48D mutation; preferably the scaffold protein comprises a G4W mutation. Preferably the scaffold comprises Leu73, V48D and G4W mutations. The invention also relates to the scaffold proteins themselves, in particular a stefin A polypeptide having the Leu73, V48D and G4W mutations, such as shown as SEQ ID NO: 1. The invention also relates to a method for identifying binding proteins and to peptide A (RLNKPLPSLPV) and its use in treating yeast infections.

摘要翻译： 本发明涉及使用Stefin A作为展示插入肽的支架蛋白，特别是其中Stefin A是人SteFen A.几种突变有利地在野生型stefin A序列中制成，以改善其作为支架 ; 优选地，Stefin A包含在Leu 73位点处的异源肽插入。 此外，优选地，支架蛋白包含V48D突变; 优选地，支架蛋白包含G4W突变。 优选地，所述支架包含Leu73，V48D和G4W突变。 本发明还涉及支架蛋白本身，特别是具有Leu73，V48D和G4W突变的stefin A多肽，如SEQ ID NO：1所示。本发明还涉及鉴定结合蛋白和肽A的方法 （RLNKPLPSLPV）及其在治疗酵母菌感染中的应用。